Evaluation of antibacterial efficacy of garlic (Allium sativum) and ginger (Zingiber officinale) crude extract against multidrug-resistant (MDR) poultry pathogen

Zakaria Al Noman; Tasnia Tabassum Anika; Sabbya Sachi; Jannatul Ferdous; Yousuf Ali Sarker; Md Abdus Sabur; Md Tanvir Rahman; Mahmudul Hasan Sikder

doi:10.5455/javar.2023.j664

Evaluation of antibacterial efficacy of garlic (Allium sativum) and ginger (Zingiber officinale) crude extract against multidrug-resistant (MDR) poultry pathogen

J Adv Vet Anim Res. 2023 Jun 30;10(2):151-156. doi: 10.5455/javar.2023.j664. eCollection 2023 Jun.

Authors

Zakaria Al Noman¹, Tasnia Tabassum Anika¹, Sabbya Sachi¹, Jannatul Ferdous¹, Yousuf Ali Sarker¹, Md Abdus Sabur², Md Tanvir Rahman², Mahmudul Hasan Sikder¹

Affiliations

¹ Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
² Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.

Abstract

Objective: The study is aimed to understand the antibacterial sensitivity of native and Indian varieties of garlic (Allium sativum) and ginger (Zingiber officinale) crude extracts against multidrug-resistant (MDR) poultry pathogen (Escherichia coli and Salmonella sp.).

Materials and methods: Thin layer chromatography (TLC) is used to identify the target spices' bioactive antibacterial compounds. MDR E. coli and Salmonella sp. were isolated from poultry. The TLC-Bioautography technique was applied to explore the antibacterial potentiality of garlic and ginger.

Results: Inhibitory activities of garlic were Zone of inhibition (ZI) = 14.03 ± 0.15 mm and 19.70 ± 0.36 mm, Minimum inhibitory concentration (MIC): 0.625 and 0.325 mg/ml, and ginger were ZI = 14.63 ± 0.30 mm and 11.56 ± 0.51mm, MIC: 9.0 mg/ml against E. coli and Salmonella sp., respectively. Two bands of garlic (R_f value = 0.31 and 0.50) and one band of ginger (R_f value = 0.71) showed inhibitory potential in TLC-Bioautography against both MDR isolates.

Conclusion: Garlic and ginger were effective against MDR E. coli and Salmonella sp. These spices could be a suitable alternative during the antibiotic void.

Keywords: Multidrug-resistant pathogen; TLC-Bioautography; alternative antibiotic.