Disruption of the cytochrome CYP711A5 gene reveals MAX1 redundancy in rice strigolactone biosynthesis

Jian You Wang; Guan-Ting Erica Chen; Justine Braguy; Muhammad Jamil; Lamis Berqdar; Salim Al-Babili

doi:10.1016/j.jplph.2023.154057

Disruption of the cytochrome CYP711A5 gene reveals MAX1 redundancy in rice strigolactone biosynthesis

J Plant Physiol. 2023 Aug:287:154057. doi: 10.1016/j.jplph.2023.154057. Epub 2023 Jul 22.

Authors

Jian You Wang¹, Guan-Ting Erica Chen², Justine Braguy¹, Muhammad Jamil¹, Lamis Berqdar¹, Salim Al-Babili³

Affiliations

¹ The BioActives Lab, Center for Desert Agriculture, King Abdullah University of Science and Technology, Saudi Arabia.
² The BioActives Lab, Center for Desert Agriculture, King Abdullah University of Science and Technology, Saudi Arabia; The Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Saudi Arabia.
³ The BioActives Lab, Center for Desert Agriculture, King Abdullah University of Science and Technology, Saudi Arabia; The Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Electronic address: salim.babili@kaust.edu.sa.

PMID: 37531662
DOI: 10.1016/j.jplph.2023.154057

Abstract

Strigolactones (SLs) inhibit shoot branching/tillering and are secreted by plant roots as a signal to attract symbiotic mycorrhizal fungi in the rhizosphere, particularly under phosphate starvation. However, SLs are also hijacked by root parasitic weeds as inducer for the germination of their seeds. There are around 35 natural SLs divided, based on their structures, into canonical and non-canonical SLs. Cytochrome P450 enzymes of the 711 clade, such as MORE AXILLARY GROWTH1 (MAX1) in Arabidopsis, are a major driver of SL structural diversity. Monocots, such as rice, contain several MAX1 homologs that participate in SL biosynthesis. To investigate the function of OsMAX1-1900 in planta, we generated CRISPR/Cas9 mutants disrupted in the corresponding gene. Characterizing of the generated mutants at metabolite and phenotype level suggests that OsMAX1-1900 loss-of-function does neither affect the SL pattern nor rice architecture, indicating functional redundancy among rice MAX1 homologs.

Keywords: Cytochrome P450; MORE AXILLARY GROWTH1; Plant architecture; Striga; Strigolactones.

MeSH terms

Arabidopsis* / genetics
Cytochrome P-450 Enzyme System / genetics
Cytochrome P-450 Enzyme System / metabolism
Lactones / metabolism
Oryza* / genetics
Oryza* / metabolism
Plant Growth Regulators / metabolism

Substances

GR24 strigolactone
Plant Growth Regulators
Lactones
Cytochrome P-450 Enzyme System