Optimization of a Validated Protocol That Determines Bioavailable Fluoride in Toothpastes

Luis Fernando Bandeira Miranda; Cinthia Pereira Machado Tabchoury; Jaime Aparecido Cury

doi:10.1159/000533154

Optimization of a Validated Protocol That Determines Bioavailable Fluoride in Toothpastes

Caries Res. 2023;57(5-6):619-624. doi: 10.1159/000533154. Epub 2023 Aug 1.

Authors

Luis Fernando Bandeira Miranda¹, Cinthia Pereira Machado Tabchoury¹, Jaime Aparecido Cury¹

Affiliation

¹ Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.

PMID: 37527627
DOI: 10.1159/000533154

Abstract

A validated protocol used to determine how much of total fluoride is bioavailable in toothpaste formulations was evaluated if it could be simplified regarding the following parameters: (1) concentration of the slurry (0.25-4%), (2) centrifugation speed (1,000-12,000 g) and time (1-10 min), and (3) incubation time for FPO32- ion hydrolysis and insoluble-F dissolution (15-60 min). Fresh and aged Na2FPO3/CaCO3 and fresh NaF/SiO2-based toothpastes were analyzed (n = 9). The improvements were the centrifugation at 1,000 g for 5 min is enough to separate insoluble-F and the time for FPO32- ion hydrolysis and insoluble-F dissolution can be shortened to 30 min.

Keywords: Available fluoride; Fluoride; Fluoride analysis; Toothpastes.

Publication types

News
Research Support, Non-U.S. Gov't

MeSH terms

Aged
Calcium Carbonate
Fluorides*
Humans
Silicon Dioxide
Sodium Fluoride
Toothpastes*

Substances

Fluorides
Toothpastes
Sodium Fluoride
Silicon Dioxide
Calcium Carbonate