Draft genome sequence of Lactobacillus helveticus OSU-BDGOAK2 and Lactobacillus kefiranofaciens OSU-BDGOA1, kefir grain isolates with potential antibacterial activity

Brianda D González-Orozco; Tasha M Santiago-Rodriguez; Israel García-Cano; Rafael Jiménez-Flores; Valente B Alvarez

doi:10.1128/MRA.00304-23

Draft genome sequence of Lactobacillus helveticus OSU-BDGOAK2 and Lactobacillus kefiranofaciens OSU-BDGOA1, kefir grain isolates with potential antibacterial activity

Microbiol Resour Announc. 2023 Sep 19;12(9):e0030423. doi: 10.1128/MRA.00304-23. Epub 2023 Aug 1.

Authors

Brianda D González-Orozco¹, Tasha M Santiago-Rodriguez², Israel García-Cano³, Rafael Jiménez-Flores¹, Valente B Alvarez¹

Affiliations

¹ Department of Food Science and Technology, The Ohio State University , Columbus, Ohio, USA.
² Diversigen, Inc. , New Brighton, Minnesota, USA.
³ Department of Food Sciences and Technology, National Institute of Medical Sciences and Nutrition Salvador Zubirán , Mexico City, Mexico.

Abstract

We present the draft genome sequence and assembly of Lactobacillus helveticus OSU-BDGOAK2 and Lactobacillus kefiranofaciens OSU-BDGOA1 isolated from kefir grains that exhibited in vitro antibacterial activity against Escherichia coli ATCC 25922, Listeria innocua ATCC 51742, and Staphylococcus epidermidis ATCC 1222. Genome analysis of both strains revealed gene clusters encoding bacteriocins.

Keywords: antibacterial activity; kefir; probiotics.

Abstract

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