A novel electrochemical aptasensor based on eco-friendly synthesized titanium dioxide nanosheets and polyethyleneimine grafted reduced graphene oxide for ultrasensitive and selective detection of ciprofloxacin

Yangguang Zhu; Xiufen Li; Mengfan Wu; Mingjiao Shi; Qichen Tian; Li Fu; Hsu-Sheng Tsai; Wan-Feng Xie; Guosong Lai; Gang Wang; Nan Jiang; Chen Ye; Cheng-Te Lin

doi:10.1016/j.aca.2023.341607

A novel electrochemical aptasensor based on eco-friendly synthesized titanium dioxide nanosheets and polyethyleneimine grafted reduced graphene oxide for ultrasensitive and selective detection of ciprofloxacin

Anal Chim Acta. 2023 Sep 22:1275:341607. doi: 10.1016/j.aca.2023.341607. Epub 2023 Jul 10.

Authors

Yangguang Zhu¹, Xiufen Li², Mengfan Wu³, Mingjiao Shi³, Qichen Tian³, Li Fu⁴, Hsu-Sheng Tsai⁵, Wan-Feng Xie⁶, Guosong Lai⁷, Gang Wang⁸, Nan Jiang⁹, Chen Ye¹⁰, Cheng-Te Lin¹¹

Affiliations

¹ Laboratory of Environmental Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, 214122, China; Qianwan Institute, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China.
² Laboratory of Environmental Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi, 214122, China. Electronic address: xfli@jiangnan.edu.cn.
³ Qianwan Institute, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China.
⁴ College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, 310018, China.
⁵ Laboratory for Space Environment and Physical Sciences, Harbin Institute of Technology, Harbin, 150001, China.
⁶ College of Electronics and Information, University-Industry Joint Center for Ocean Observation and Broadband Communication, Qingdao University, Qingdao, 266071, China.
⁷ Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, China.
⁸ Department of Microelectronic Science and Engineering, School of Physical Science and Technology, Ningbo University, Ningbo, 315211, China.
⁹ Qianwan Institute, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China; Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China.
¹⁰ Qianwan Institute, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China; Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China. Electronic address: yechen@nimte.ac.cn.
¹¹ Qianwan Institute, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China; Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China; Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering (NIMTE), Chinese Academy of Sciences, Ningbo, 315201, China. Electronic address: linzhengde@nimte.ac.cn.

PMID: 37524471
DOI: 10.1016/j.aca.2023.341607

Abstract

Developing a rapid, sensitive, and efficient analytical method for the trace-level determination of highly concerning antibiotic ciprofloxacin (CIP) is desirable to guarantee the safety of human health and ecosystems. In this work, a novel electrochemical aptasensor based on polyethyleneimine grafted reduced graphene oxide and titanium dioxide (rGO/PEI/TiO₂) nanocomposite was constructed for ultrasensitive and selective detection of CIP. Through the in-situ electrochemical oxidation of Ti₃C₂T_x nanosheets, TiO₂ nanosheets with good electrochemical response were prepared in a more convenient and eco-friendly method. The prepared TiO₂ nanosheets promote charge transferring on electrode interface, and [Fe(CN)₆]^3-/4- as electrochemical active substance can be electrostatically attracted by rGO/PEI. Thus, electrochemical detection signal of the aptasensor variates a lot after specific binding with CIP, achieving working dynamic range of 0.003-10.0 μmol L^-1, low detection limit down to 0.7 nmol L^-1 (S/N = 3) and selectivity towards other antibiotics. Additionally, the aptasensor exhibited good agreement with HPLC method at 95% confidence level, and achieved good recoveries (96.8-106.3%) in real water samples, demonstrating its suitable applicability of trace detection of CIP in aquatic environment.

Keywords: Ciprofloxacin; Electrochemical aptasensor; Interface charge transferring; Polyethyleneimine; Titanium dioxide nanosheets.

MeSH terms

Anti-Bacterial Agents
Aptamers, Nucleotide* / chemistry
Biosensing Techniques* / methods
Ciprofloxacin
Ecosystem
Electrochemical Techniques / methods
Graphite* / chemistry
Humans
Limit of Detection
Polyethyleneimine
Titanium / chemistry

Substances

graphene oxide
titanium dioxide
Polyethyleneimine
Ciprofloxacin
Aptamers, Nucleotide
Graphite
Titanium
Anti-Bacterial Agents