Protein kinase Cβ is involved in cigarette smoke gas phase-induced ferroptosis in J774 macrophages

Tsunehito Higashi; Haruka Handa; Yosuke Mai; Katsumi Maenaka; Takashi Tadokoro

doi:10.1016/j.jphs.2023.06.001

Protein kinase Cβ is involved in cigarette smoke gas phase-induced ferroptosis in J774 macrophages

J Pharmacol Sci. 2023 Sep;153(1):22-25. doi: 10.1016/j.jphs.2023.06.001. Epub 2023 Jun 21.

Authors

Tsunehito Higashi¹, Haruka Handa², Yosuke Mai³, Katsumi Maenaka⁴, Takashi Tadokoro⁵

Affiliations

¹ Department of Cellular Pharmacology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, West 7, North 15, Kita-ku, Sapporo, Hokkaido 060-8638, Japan. Electronic address: thigashi@med.hokudai.ac.jp.
² Department of Molecular Biology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, West 7, North 15, Kita-ku, Sapporo, Hokkaido 060-8638, Japan.
³ Department of Dermatology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, West 7, North 15, Kita-ku, Sapporo, Hokkaido 060-8638, Japan.
⁴ Laboratory of Biomolecule Science, Faculty of Pharmaceutical Sciences, Hokkaido University, West 6, North 12, Kita-ku, Sapporo, Hokkaido, 060-0812, Japan; Center for Research and Education on Drug Discovery, Faculty of Pharmaceutical Sciences, Hokkaido University, West 6, North 12, Kita-ku, Sapporo, Hokkaido, 060-0812, Japan; Hokkaido University Institute for Vaccine Research & Development, West 6, North 12, Kita-ku, Sapporo, Hokkaido, 060-0812, Japan; Global Station for Biosurfaces and Drug Discovery, Hokkaido University, West 6, North 12, Kita-ku, Sapporo, Hokkaido, 060-0812, Japan.
⁵ Center for Research and Education on Drug Discovery, Faculty of Pharmaceutical Sciences, Hokkaido University, West 6, North 12, Kita-ku, Sapporo, Hokkaido, 060-0812, Japan; Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, 1-1-1 Daigakudori, Sanyo-Onoda, Yamaguchi, 756-0884, Japan.

PMID: 37524450
DOI: 10.1016/j.jphs.2023.06.001

Abstract

Cigarette smoking is a risk factor for respiratory infection caused by immune cell dysfunction. Cigarette smoke is divided into tar and gas phases. Although the gas phase induces cell death in various cell types, the mechanism for gas phase-induced cell death remains to be clarified. In this study, we have examined the effects of cigarette smoke gas phase on J774 macrophages. Cigarette smoke gas phase and cytotoxic factors in the gas phase induced protein kinase C (PKC)-dependent ferroptosis. Pharmacological studies using isoform-specific PKC inhibitors have revealed that PKCβ is involved in cigarette smoke gas phase-induced ferroptosis in J774 macrophages.

Keywords: Cigarette smoke gas phase; Ferroptosis; Protein kinase C.