Use of platelet lysate for in-vitro embryo production and treatment of repeat breeding in cows

Ahmed Saad Ahmed Hassaneen; Mohamed Sadawy Rawy; Eigo Yamanokuchi; Omnia Elgendy; Takanori Kawano; Shoichi Wakitani; Go Kitahara; Takeshi Osawa

doi:10.1016/j.theriogenology.2023.07.034

Use of platelet lysate for in-vitro embryo production and treatment of repeat breeding in cows

Theriogenology. 2023 Oct 15:210:199-206. doi: 10.1016/j.theriogenology.2023.07.034. Epub 2023 Jul 25.

Authors

Ahmed Saad Ahmed Hassaneen¹, Mohamed Sadawy Rawy², Eigo Yamanokuchi³, Omnia Elgendy⁴, Takanori Kawano⁵, Shoichi Wakitani⁶, Go Kitahara⁷, Takeshi Osawa⁸

Affiliations

¹ Department of Theriogenology, Obstetrics, and Artificial Insemination, Faculty of Veterinary Medicine, South Valley University, 83523, Qena, Egypt; Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. Electronic address: ahmed.hassaneen@vet.svu.edu.eg.
² Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan; Department of Theriogenology, Faculty of Veterinary Medicine, Minia University, Minia, Egypt. Electronic address: sadawyvet84@yahoo.com.
³ Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. Electronic address: a5orfevre@gmail.com.
⁴ Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan; Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Qalyobia, 13736, Egypt. Electronic address: omniavet@yahoo.com.
⁵ Miyazaki Agricultural Mutual Aid Association, Shintomi, Miyazaki, 889-1406, Japan. Electronic address: t.kawano@nosai-miyazakiken.jp.
⁶ Laboratory of Veterinary Anatomy, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. Electronic address: show1@cc.miyazaki-u.ac.jp.
⁷ Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. Electronic address: gkitahara@cc.miyazaki-u.ac.jp.
⁸ Laboratory of Theriogenology, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, 889-2192, Japan. Electronic address: osawa@cc.miyazaki-u.ac.jp.

PMID: 37523941
DOI: 10.1016/j.theriogenology.2023.07.034

Abstract

Platelet-rich plasma (PRP) is a biological hemocomponent derived from blood after the complete removal of red blood cells and the partial or complete removal of white blood cells to concentrate platelets in an appropriate volume of plasma. Platelets have important growth factors, cytokines, and active metabolites that improve the endometrial environment and positively affect implantation. This study evaluated the effect of the addition of activated PRP (platelets lysate; PL) on in vitro bovine oocyte maturation and embryonic development and the effect of intrauterine (IU) infusion of autologous PL in repeat breeder (RB) cows. Experiment 1 examined the effects of allogeneic PL, fetal calf serum (FCS), mixed PL + FCS, or platelet-poor plasma (PPP) supplementations to in vitro maturation and development media on in vitro oocyte maturation and embryo development in good- and poor-quality oocytes of Japanese Black cows. Experiment 2 examined the IU infusion of autologous PL, 24 h post-insemination, in 21 RB Holstein-Friesian dairy cows. The cleavage rate of good-quality oocytes was higher in the PL group (85.93 ± 2.50%) than in the PPP group (67.16 ± 3.41%) (P < 0.05), while the cleavage rate of the poor-quality oocytes was higher in the PL alone (76.13 ± 4.04%) and mixed PL + FCS treated (73.59 ± 4.22%) groups than in the PPP group (54.64 ± 2.93%) (P < 0.05). The blastocyst rate of the good-quality oocytes was higher in the PL group (40.97 ± 3.03%) than in the FCS (27.97 ± 3.31%) and PPP (25.33 ± 2.15%) groups (P < 0.05). The blastocyst rate of poor-quality oocytes and the hatching rates of both good and poor-quality oocytes showed no significant differences among all groups. The conception rate in the autologous PL-treated group was 41.67% (5/12), while it was 11.11% (1/9) in the control group. The platelets' count in the pregnant PL-treated cows (n = 5; mean ± SEM, 1.07 ± 0.10 × 10⁹/mL) was higher than in the non-pregnant ones (n = 7; 0.67 ± 0.10 × 10⁹/mL) (P < 0.05). In conclusion, allogeneic PL was effective in stimulating the in vitro oocyte maturation and embryonic development in both good and poor-quality bovine oocytes, and post-insemination IU infusion of autologous PL derived from high platelets' count-PRP would be recommended for the treatment of RB cows.

Keywords: Cows; Embryo development; In vitro oocyte maturation; Platelet-rich plasma; Repeat breeder.

MeSH terms

Animals
Cattle
Embryonic Development
Female
Fertilization in Vitro / veterinary
Fertilization*
In Vitro Oocyte Maturation Techniques / veterinary
Oocytes*
Pregnancy