Dietary total antioxidant capacity interacts with a variant of chromosome 5q13-14 locus to influence cardio-metabolic risk factors among obese adults

Mahdieh Khodarahmi; Amir Sobhrakhshan Khah; Mahdieh Abbasalizad Farhangi; Goli Siri; Houman Kahroba

doi:10.1186/s43042-022-00328-3

Dietary total antioxidant capacity interacts with a variant of chromosome 5q13-14 locus to influence cardio-metabolic risk factors among obese adults

Egypt J Med Hum Genet. 2022;23(1):117. doi: 10.1186/s43042-022-00328-3. Epub 2022 Aug 5.

Authors

Mahdieh Khodarahmi¹, Amir Sobhrakhshan Khah², Mahdieh Abbasalizad Farhangi³, Goli Siri⁴, Houman Kahroba^{5

6}

Affiliations

¹ Department of Community Nutrition, Faculty of Nutrition and Food Science, Tabriz University of Medical Sciences, Tabriz, Iran.
² Sepehr Heart Center, Baharloo Hospital, Tehran University of Medical Sciences, Tehran, Iran.
³ Drug Applied Research Center, Tabriz University of Medical Sciences, Attar-neishabouri Ave, Golgasht St, Tabriz, 5165665931 Iran.
⁴ Department of Internal Medicine, Amir-Alam Hospital, Tehran University of Medical Sciences, Tehran, Iran.
⁵ Department of Toxicogenomics, GROW School of Oncology and Development Biology, Maastricht University, Maastricht, The Netherlands.
⁶ Centre for Environmental Sciences, Hasselt University, Hasselt, Belgium.

Abstract

Background: The association between cocaine- and amphetamine-regulated transcript prepropeptide gene (CARTPT) and obesity-related outcomes has shown in the epidemiological studies. Nevertheless, there is lack of data regarding the CARTPT gene-diet interactions in terms of antioxidant potential of diet. So, this study aimed to test CARTPT gene-dietary non-enzymatic antioxidant capacity (NEAC) interactions on cardio-metabolic risk factors in obese individuals.

Methods and material: The present cross-sectional study was carried out among 288 apparently healthy obese adults within age range of 20-50 years. Antioxidant capacity of diet was estimated by calculating the oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), total radical-trapping antioxidant parameter (TRAP) and Trolox equivalent antioxidant capacity (TEAC) using a semiquantitative food frequency questionnaire (FFQ). Genotyping for CARTPT rs2239670 polymorphism was conducted by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

Results: A significant interaction was revealed between CARTPT rs2239670 and dietary ORAC on BMI (P_Interaction = 0.048) and fat mass percent (FM%) (P_Interaction = 0.008); in A allele carriers, higher adherence to the dietary ORAC was related to lower level of BMI and FM%. And, the significant interactions were observed between FRAP index and rs2239670 in relation to HOMA (P_Interaction = 0.049) and QUICKI (P_Interaction = 0.048). Moreover, there were significant interactions of rs2239670 with TRAP (P_Interaction = 0.029) and TEAC (P_Interaction = 0.034) on the serum glucose level; individuals with AG genotype were more respondent to higher intake of TRAP.

Conclusion: The present study indicated that the relationships between CARTPT rs2239670 and obesity and its-related metabolic parameters depend on adherence to the dietary NEAC. Large prospective studies are needed to confirm our findings.

Keywords: CARTPT; Gene–diet interaction; Metabolic factors; Obesity; Polymorphism; Total antioxidant capacity.