Novel sensor for the determination of CA 15-3 in serum of breast cancer patients based on Fe-gallic acid complex doped in modified cellulose polymer thin films

Hind A AlGhamdi; Yasmeen M AlZahrani; Salha Alharthi; Mohamed S Mohy-Eldin; Ekram H Mohamed; Safwat A Mahmoud; Mohamed S Attia

doi:10.1039/d3ra02495d

Novel sensor for the determination of CA 15-3 in serum of breast cancer patients based on Fe-gallic acid complex doped in modified cellulose polymer thin films

RSC Adv. 2023 Jul 28;13(31):21769-21780. doi: 10.1039/d3ra02495d. eCollection 2023 Jul 12.

Authors

Hind A AlGhamdi¹, Yasmeen M AlZahrani¹, Salha Alharthi¹, Mohamed S Mohy-Eldin², Ekram H Mohamed³, Safwat A Mahmoud⁴, Mohamed S Attia⁵

Affiliations

¹ Chemistry Department, College of Science, Imam Abdulrahman Bin Faisal University P.O. Box 1982 Dammam 31441 Saudi Arabia.
² Polymer Materials Research Department, Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientifc Research and Technological Applications (SRTA-City) New Borg El-Arab City, P. O. Box: 21934 Alexandria Egypt.
³ Analytical Chemistry Department, The British University in Egypt El Sherouk city Cairo 11378 Egypt.
⁴ Physics Department, Faculty of Science, Northern Border University Arar Saudi Arabia.
⁵ Chemistry Department, Faculty of Science, Ain Shams University Abbassia Cairo 11566 Egypt Mohamed_sam@yahoo.com.

Abstract

Fe-gallic acid MOF embedded in an epoxy methyl cellulose polymer (CMC) thin film was synthesized and characterized by different micro-analytical tools such as: FE-SEM/EDX, XPS analysis, XRD analysis, FT-IR, and fluorescence spectroscopy. Fe-gallic acid MOF doped in a stable CMC polymer thin film is used as a novel sensor to identify CA 15-3 in the sera of patients suffering breast malignancy. The presence of appropriate functional groups in aqueous CA 15-3 solutions enables it to interact with the Fe-gallic acid MOF embedded in the thin film. The Fe-gallic acid MOF was found to absorb energy at 350 nm (λ_ex) and emits radiation at 439 nm which was specifically quenched in the presence of CA 15-3 over a working concentration range of 0.05-570 U mL^-1. In contrast to other CA 15-3 detection methods which suffered from electronic noise, interference and slowness, the Fe-gallic acid MOF proved its sensitivity as an economic, stable and reliable probe for the detection and determination of CA 15-3 in patients' serum samples with a detection limit of 0.01 U mL^-1 at pH 7.2.