ANXA13 promotes cell proliferation and invasion and attenuates apoptosis in renal cell carcinoma

Xiaoyu Niu; Keyuan Zhao; Yuanyuan Zheng; Yapeng Wang; Ruoyang Liu; Yiming Zhang; Lihui Wang; Yongjun Wu; Xuefeng Bai; Baoping Qiao

doi:10.1016/j.heliyon.2023.e18009

ANXA13 promotes cell proliferation and invasion and attenuates apoptosis in renal cell carcinoma

Heliyon. 2023 Jul 22;9(8):e18009. doi: 10.1016/j.heliyon.2023.e18009. eCollection 2023 Aug.

Authors

Xiaoyu Niu¹, Keyuan Zhao², Yuanyuan Zheng³, Yapeng Wang⁴, Ruoyang Liu¹, Yiming Zhang¹, Lihui Wang¹, Yongjun Wu^{5

6}, Xuefeng Bai⁶, Baoping Qiao¹

Affiliations

¹ Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China.
² Department of Urology, Shaoxing People's Hospital, Shaoxing, Zhejiang, 312000, China.
³ National Engineering Laboratory for Internet Medical Systems and Applications, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China.
⁴ Department of Urology, Daping Hospital, Army Medical University, Chongqing, 404100, China.
⁵ College of Public Health, Zhengzhou University, Zhengzhou, Henan, 450001, China.
⁶ The Key Laboratory of Nanomedicine and Health Inspection of Zhengzhou, Zhengzhou, Henan, 450001, China.

Abstract

Purpose: Emerging evidences have demonstrated that annexin A13 (ANXA13) is closely related to the occurrence and development of malignant tumors. However, the functions and underlying molecular mechanisms of ANXA13 in Clear cell renal cell carcinoma (ccRCC) have not been defined. Therefore, this study aimed to clarify the potential role of ANXA13 in regulating the proliferation, migration, invasion, cell cycle, and apoptosis of ccRCC cells.

Patients and methods: The quantitative real-time PCR (qRT-PCR) and western blotting was performed for detecting the ANXA13 expression in ccRCC tissues at the mRNA and protein levels, respectively. The GEPIA2 databases were used to derive data for analyzing the ANXA13 expression in pan-cancer and ccRCC clinical features. Cell Counting and colony formation assays, as well as flow cytometry, were used to detect cell proliferation, apoptosis, or cell cycle. The wound healing assay was used to evaluate the migration ability of cells, and the Trans-well assay was conducted to determine the cell invasiveness.

Results: ANXA13 was upregulated in ccRCC cells and human ccRCC tissues. Furthermore, siANXA13 inhibited ccRCC cell proliferation, migration, invasion and induced cell apoptosis.

Conclusion: ANXA13 was upregulated in ccRCC. ANXA13 promotes tumorigenic traits of ccRCC cell lines in vitro. ANXA13 is a potential novel biomarker and a potential therapeutic target in ccRCC.

Keywords: ANXA13; Apoptosis; Invasion and metastasis; Proliferation; ccRCC.