Cloning, purification and characterization of human dentine matrix protein 1(DMP1)

Tarek El-Bialy; Dror Aizenbud; Hamid Sadeghi; Paul Scott; Itay Aizenbud

doi:10.1016/j.sdentj.2023.05.006

Cloning, purification and characterization of human dentine matrix protein 1(DMP1)

Saudi Dent J. 2023 Jul;35(5):553-558. doi: 10.1016/j.sdentj.2023.05.006. Epub 2023 May 19.

Authors

Tarek El-Bialy¹, Dror Aizenbud², Hamid Sadeghi³, Paul Scott⁴, Itay Aizenbud⁵

Affiliations

¹ Division Orthodontics, School of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada.
² Department of Orthodontics and Craniofacial Anomalies, School of Graduate Dentistry, Rambam Health Care Center and Technion Haifa, Israel.
³ Department of Dentistry, University of Alberta, Edmonton, Canada.
⁴ Department of Biochemistry, University of Alberta, Edmonton Canada.
⁵ Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University, Jerusalem; Medical Corps, Israel Defense Forces, Tel-Hashomer, Israel.

Abstract

Introduction: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1.

Materials and methods: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells.

Results: The recombinant DMP1 was purified and characterized.

Conclusion: The results suggested its future use in dental tissue regeneration and tissue engineering.

Keywords: Cloning; Dentine matrix protein; Human; Purification.