Amplification of microbial DNA from bacterial extracellular vesicles from human placenta

Ramkumar Menon; Kamil Khanipov; Enkhtuya Radnaa; Esha Ganguly; Giovana Fernanda Cosi Bento; Rheanna Urrabaz-Garza; Ananth Kumar Kammala; Jerome Yaklic; Richard Pyles; George Golovko; Ourlad Alzeus G Tantengco

doi:10.3389/fmicb.2023.1213234

Amplification of microbial DNA from bacterial extracellular vesicles from human placenta

Front Microbiol. 2023 Jul 13:14:1213234. doi: 10.3389/fmicb.2023.1213234. eCollection 2023.

Affiliations

¹ Division of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United States.
² Department of Pharmacology and Toxicology, The University of Texas Medical Branch at Galveston, Galveston, TX, United States.
³ Department of Pediatrics, The University of Texas Medical Branch at Galveston, Galveston, TX, United States.
⁴ Department of Physiology, College of Medicine, University of the Philippines Manila, Manila, Philippines.
⁵ Department of Biology, College of Science, De La Salle University, Manila, Philippines.

Abstract

Introduction: The placenta is essential for fetal growth and survival and maintaining a successful pregnancy. The sterility of the placenta has been challenged recently; however, the presence of a placental microbiome has been controversial. We tested the hypothesis that the bacterial extracellular vesicles (BEVs) from Gram-negative bacteria as an alternate source of microbial DNA, regardless of the existence of a microbial community in the placenta.

Methods: Placentae from the term, not in labor Cesareans deliveries, were used for this study, and placental specimens were sampled randomly from the fetal side. We developed a protocol for the isolation of BEVs from human tissues and this is the first study to isolate the BEVs from human tissue and characterize them.

Results: The median size of BEVs was 130-140 nm, and the mean concentration was 1.8-5.5 × 10¹⁰ BEVs/g of the wet placenta. BEVs are spherical and contain LPS and ompA. Western blots further confirmed ompA but not human EVs markers ALIX confirming the purity of preparations. Taxonomic abundance profiles showed BEV sequence reads above the levels of the negative controls (all reagent controls). In contrast, the sequence reads in the same placenta were substantially low, indicating nothing beyond contamination (low biomass). Alpha-diversity showed the number of detected genera was significantly higher in the BEVs than placenta, suggesting BEVs as a likely source of microbial DNA. Beta-diversity further showed significant overlap in the microbiome between BEV and the placenta, confirming that BEVs in the placenta are likely a source of microbial DNA in the placenta. Uptake studies localized BEVs in maternal (decidual) and placental cells (cytotrophoblast), confirming their ability to enter these cells. Lastly, BEVs significantly increased inflammatory cytokine production in THP-1 macrophages in a high-dose group but not in the placental or decidual cells.

Conclusion: We conclude that the BEVs are normal constituents during pregnancy and likely reach the placenta through hematogenous spread from maternal body sites that harbor microbiome. Their presence may result in a low-grade localized inflammation to prime an antigen response in the placenta; however, insufficient to cause a fetal inflammatory response and adverse pregnancy events. This study suggests that BEVs can confound placental microbiome studies, but their low biomass in the placenta is unlikely to have any immunologic impact.

Keywords: EVS; bacterial outer membrane vesicles; metagenomics; parturition; placenta; pregnancy.