The prevalence and molecular detection of bovine cysticercosis and its impact on slaughtered cattle in Egypt

Khaled Mohamed El-Dakhly; Shaimaa Ahmed Hany; Waleed M Arafa; Omima Ramadan Abdel-Fatah; Nasser Sayed Abdel-Atty; El-Shaymaa El-Nahass

doi:10.1007/s12639-023-01596-5

The prevalence and molecular detection of bovine cysticercosis and its impact on slaughtered cattle in Egypt

J Parasit Dis. 2023 Sep;47(3):527-534. doi: 10.1007/s12639-023-01596-5. Epub 2023 May 18.

Authors

Khaled Mohamed El-Dakhly¹, Shaimaa Ahmed Hany², Waleed M Arafa¹, Omima Ramadan Abdel-Fatah³, Nasser Sayed Abdel-Atty⁴, El-Shaymaa El-Nahass⁵

Affiliations

¹ Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511 Egypt.
² Fayoum University Hospitals, El Fayoum, 63522 Egypt.
³ Animal Health Research Institute, El Fayoum, 63522 Egypt.
⁴ Department of Food Safety and Technology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511 Egypt.
⁵ Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511 Egypt.

PMID: 37520208
PMCID: PMC10382426 (available on 2024-09-01)
DOI: 10.1007/s12639-023-01596-5

Abstract

Cysticercus bovis is the metacestode of the commonly defined foodborne cestode, Taenia saginata. It infects heart, masseter muscles and other muscular sites of cattle causing bovine cysticercosis. So, a retrospective study using archival documents from authorized agencies is done during the period of 2018-2020 with estimating economic losses. Cysts were collected from municipal Beni-Suef and Basatin abattoirs. Molecular approach including cPCR and sequencing analysis is used to prove the species. The prevalence was 0.34% in Beni-Suef abattoir and 5.70% in Basatin abattoir including heads, hearts or all carcasses condemned. Among 27 provinces surveyed, the highest prevalence was recorded in Aswan followed by Cairo, Suez and Red Sea. Moreover, 19 provinces (Cairo, Alexandria, Suez, Dakahlia, Sharkia, Qalyobia, Kafrelsheikh, Gharbia, Menofiya, Ismailia, Giza, Beni-Suef, Assiut, Sohag, Qena, Aswan, Red Sea, South Sinai and El-Wadi El-Gadid) recorded bovine cysticercosis in 2018, 2019 and 2020. On the other hand, Luxor and North Sinai had no infections. The percentages of condemnation among slaughtered cattle were 1.38, 1.49 and 0.87% in 2018, 2019 and 2020, respectively. Condemnations significantly varied among north, middle and south districts of Egypt, and also varied annually. Molecularly, a diagnostic band at an amplicon size 253 bp targeting the COI gene specific for Cycticercus bovis was revealed. The obtained sequences showed 100% identity with the different Taenia saginata COI GB sequence isolates in many countries worldwide. The phylogenetic analysis method showed that the obtained sequences originating from the same clade of Taenia saginata GB isolates globally. Careful meat inspection as well as strict hygienic measures is recommended for both veterinarians and public.

Keywords: Cysticercus bovis; Egypt; PCR; Prevalence; Sequencing.

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