Spatial topology of organelle is a new breast cancer cell classifier

Ling Wang; Joshua Goldwag; Megan Bouyea; Jonathan Barra; Kailie Matteson; Niva Maharjan; Amina Eladdadi; Mark J Embrechts; Xavier Intes; Uwe Kruger; Margarida Barroso

doi:10.1016/j.isci.2023.107229

Spatial topology of organelle is a new breast cancer cell classifier

iScience. 2023 Jun 28;26(7):107229. doi: 10.1016/j.isci.2023.107229. eCollection 2023 Jul 21.

Authors

Affiliations

¹ Department of Molecular and Cellular Physiology, Albany Medical College, Albany, NY 12208, USA.
² Department of Mathematics, The College of Saint Rose, Albany, NY 12203, USA.
³ Department of Industrial and Systems Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
⁴ Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.

Abstract

Genomics and proteomics have been central to identify tumor cell populations, but more accurate approaches to classify cell subtypes are still lacking. We propose a new methodology to accurately classify cancer cells based on their organelle spatial topology. Herein, we developed an organelle topology-based cell classification pipeline (OTCCP), which integrates artificial intelligence (AI) and imaging quantification to analyze organelle spatial distribution and inter-organelle topology. OTCCP was used to classify a panel of human breast cancer cells, grown as 2D monolayer or 3D tumor spheroids using early endosomes, mitochondria, and their inter-organelle contacts. Organelle topology allows for a highly precise differentiation between cell lines of different subtypes and aggressiveness. These findings lay the groundwork for using organelle topological profiling as a fast and efficient method for phenotyping breast cancer function as well as a discovery tool to advance our understanding of cancer cell biology at the subcellular level.

Keywords: Cancer; Cell biology; Organizational aspects of cell biology.

Abstract

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