Isolation and purification of potential weed inhibitors from Mimosa pigra L

Do Tan Khang; Tran Ngoc Quy; Nguyen Phuc Dam; Nguyen Trong Tuan; Tran Thanh Men; Nguyen Van Ay; Nguyen Phuong Thuy

doi:10.1016/j.heliyon.2023.e18205

Isolation and purification of potential weed inhibitors from Mimosa pigra L

Heliyon. 2023 Jul 17;9(7):e18205. doi: 10.1016/j.heliyon.2023.e18205. eCollection 2023 Jul.

Authors

Do Tan Khang¹, Tran Ngoc Quy¹, Nguyen Phuc Dam², Nguyen Trong Tuan³, Tran Thanh Men³, Nguyen Van Ay⁴, Nguyen Phuong Thuy⁵

Affiliations

¹ Institute of Food and Biotechnology, Can Tho University, 94000, Can Tho City, Viet Nam.
² College of Education, Can Tho University, 94000, Can Tho City, Viet Nam.
³ College of Natural Sciences, Can Tho University, 94000, Can Tho City, Viet Nam.
⁴ College of Agriculture, Can Tho University, 94000, Can Tho City, Viet Nam.
⁵ School of Agriculture and Aquaculture, Tra Vinh University, Tra Vinh province, 87000, Viet Nam.

Abstract

The diversity in structure and herbicidal properties detected in natural phytotoxic compounds could bring about advantages for development bio-herbicides. The present study was carried out search for potential weed inhibitors from the parts of Mimosa pigra L. The ethyl acetate (EtOAc) extract of leaf of M. pigra showed inhibitory activity during the time that Echinochloa crus-galli (barnyardgrass) germinates and grows, which is greater than that of other extracts. From this active extract, potent growth inhibitors were isolated and identified by column chromatography (CC), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (¹H and ¹³C NMR). The six compounds were purified in this study namely: lupeol (C1, 13.2 mg), stigmastane-3,6-dione (C2, 14.7 mg), quercetin (C3, 20.2 mg), chrysoeriol (C4, 28 mg), methyl gallate (C5, 21.5 mg) and daucosterol (C6, 16.0 mg). The C2 (quercetin) compound completely inhibited the emergency, shoot height and root length of E. crus-galli at 1 mg/mL concentration (IC₅₀ shoot height = 0.56 mg/mL). This was also the first study to report the isolation and allelopathic activity of lupeol, chrysoeriol and daucosterol from M. pigra leaf. Findings of this study highlighted that quercetin from M. pigra may become bio-herbicide to control barnyard grass and other grass weeds for the development of safe agriculture.

Keywords: Allelopathic; Bio-herbicide; Echinochloa crus-galli; Inhibitor; Mimosa pigra; Quercetin.