CRISPR-based m6A modification and its potential applications in telomerase regulation

Mingliang Yi; Mingyue Wang; Yongjie Xu; Zhikun Cao; Yinghui Ling; Zijun Zhang; Hongguo Cao

doi:10.3389/fcell.2023.1200734

CRISPR-based m⁶A modification and its potential applications in telomerase regulation

Front Cell Dev Biol. 2023 Jul 14:11:1200734. doi: 10.3389/fcell.2023.1200734. eCollection 2023.

Authors

Mingliang Yi¹, Mingyue Wang¹, Yongjie Xu¹, Zhikun Cao¹, Yinghui Ling^{1

2}, Zijun Zhang^{1

2}, Hongguo Cao^{1

2}

Affiliations

¹ Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Anhui Agricultural University, Hefei, China.
² College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.

Abstract

Telomerase determines cell lifespan by controlling chromosome stability and cell viability, m⁶A epigenetic modification plays an important role in the regulation of telomerase activity. Using CRISPR epigenome editing to analyze specific m⁶A modification sites in telomerase will provide an important tool for analyzing the molecular mechanism of m⁶A modification regulating telomerase activity. In this review, we clarified the relevant applications of CRISPR system, paid special attention to the regulation of m⁶A modification in stem cells and cancer cells based on CRISPR system, emphasized the regulation of m⁶A modification on telomerase activity, pointed out that m⁶A modification sites regulate telomerase activity, and discussed strategies based on telomerase activity and disease treatment, which are helpful to promote the research of anti-aging and tumor related diseases.

Keywords: CRISPR system; epigenetic regulation; m6A modification; telomerase; telomere.

Publication types

Review

Grants and funding

This research was partially supported by National Natural Science Foundation of China (project code: 32272875), Anhui Natural Science Foundation (project code: 2208085MC75) and Anhui Meat Sheep Joint Breeding Project (project code: 340000211260001000431).