Application of FT-IR Spectroscopy for Mycobacterium abscessus complex subspecies differentiation

Antonio Curtoni; Miriam Cordovana; Alessandro Bondi; Francesca Scaiola; Giulia Criscione; Davide Ghibaudo; Lisa Pastrone; Elisa Zanotto; Anna Camaggi; Maria Simona Caroppo; Markus Kostrzewa; Rossana Cavallo; Cristina Costa

doi:10.1016/j.mimet.2023.106792

Application of FT-IR Spectroscopy for Mycobacterium abscessus complex subspecies differentiation

J Microbiol Methods. 2023 Sep:212:106792. doi: 10.1016/j.mimet.2023.106792. Epub 2023 Jul 28.

Authors

Affiliations

¹ Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Turin, Italy; Department of Public Health and Paediatrics, University of Turin, Turin, Italy.
² Bruker Daltonics GmbH & Co. KG, Bremen, Germany.
³ Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Turin, Italy; Department of Public Health and Paediatrics, University of Turin, Turin, Italy. Electronic address: alessandro.bondi@unito.it.
⁴ Department of Public Health and Paediatrics, University of Turin, Turin, Italy.
⁵ Microbiology and Virology Unit, University Hospital Città della Salute e della Scienza di Torino, Turin, Italy.
⁶ Microbiology and Virology Unit, Azienda Ospedaliero-Universitaria Maggiore della Carità di Novara, Novara, Italy.

PMID: 37517511
DOI: 10.1016/j.mimet.2023.106792

Abstract

Mycobacterium abscessus complex (MABSC) subspecies differentiation improves patients' therapy and outcome. Fourier-Transform-Infrared Spectroscopy (FT-IRS) was applied for subspecies discrimination of 15 strains on different media: Löwenstein-Jensen showed the best resolution power; Linear Discriminant Analysis model differentiated M. abscessus susbsp. abscessus from M. abscessus subsp. massiliense. FT-IRS has a potential role in rapidly MABSC subspecies identification.

Keywords: FT-IR spectroscopy; IR biotyper; LPA; Mycobacterium abscessus complex; Subtyping.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Mycobacterium abscessus*
Spectroscopy, Fourier Transform Infrared