Tannic acid-Fe3+ dual catalysis induced rapid polymerization of injectable poly(lysine) hydrogel for infected wound healing

Wenxia Guo; Yang Li; Chenhui Zhu; Zhiguang Duan; Rongzhan Fu; Daidi Fan

doi:10.1016/j.ijbiomac.2023.125911

Tannic acid-Fe³⁺ dual catalysis induced rapid polymerization of injectable poly(lysine) hydrogel for infected wound healing

Int J Biol Macromol. 2023 Sep 30:249:125911. doi: 10.1016/j.ijbiomac.2023.125911. Epub 2023 Jul 28.

Authors

Wenxia Guo¹, Yang Li¹, Chenhui Zhu¹, Zhiguang Duan¹, Rongzhan Fu², Daidi Fan³

Affiliations

¹ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710069, Shaanxi, China.
² Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710069, Shaanxi, China. Electronic address: rongzhanfu@nwu.edu.cn.
³ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an 710069, Shaanxi, China. Electronic address: fandaidi@nwu.edu.cn.

PMID: 37516228
DOI: 10.1016/j.ijbiomac.2023.125911

Abstract

Infected wounds are difficult to heal because they are vulnerable to bacterial attacks, inflammatory responses, and oxidative stress. To promote the healing of infected wounds, we developed an injectable dual-network hydrogel TFAEP (TA-Fe, APS, EPL-GMA, PVA) based on ε-poly-l-lysine-graft-glycidyl methacrylate (EPL-GMA), polyvinyl alcohol (PVA), and tannic acid-iron (TA-Fe). TA-Fe formed a stable redox pair, which acted as a dual-autocatalytic system to activate ammonium persulfate, generate free radicals, and subsequently induce EPL-GMA polymerization. Then PVA formed hydrogen bonds with TA molecules. Here, TA-Fe not only simulated peroxidase to convert H₂O₂ into hydroxyl radicals (OH), but also exhibited good near-infrared photothermal conversion efficiency, which all endowed the hydrogel with excellent antibacterial ability. In addition, the hydrogel could remove excessive reactive oxygen species and reactive nitrogen species, alleviating oxidative stress and reducing inflammation response due to the presence of TA molecules. Moreover, the hydrogel showed good injectability and tissue adhesion, ensuring the close adhesion of the hydrogel to the wound and achieving the maximum function. In vivo experiments demonstrated that the hydrogel promoted infected wound healing by accelerating epidermal regeneration, promoting angiogenesis and collagen deposition, and facilitating the expression of anti-inflammatory factors.

Keywords: Bacterial infected wound healing; Poly(lysine) modified with glycidyl methacrylate; TA-Fe.

MeSH terms

Anti-Bacterial Agents / pharmacology
Catalysis
Hydrogels* / pharmacology
Hydrogen Peroxide*
Lysine
Polymerization
Wound Healing

Substances

Hydrogels
Tannic Acid
Hydrogen Peroxide
Lysine
Anti-Bacterial Agents