Unveiling the mechanisms underlying photothermal efficiency of gold shell-isolated nanoparticles (AuSHINs) on ductal mammary carcinoma cells (BT-474)

M B Kobal; S A Camacho; L G Moreira; K A Toledo; D B Tada; P H B Aoki

doi:10.1016/j.bpc.2023.107077

Unveiling the mechanisms underlying photothermal efficiency of gold shell-isolated nanoparticles (AuSHINs) on ductal mammary carcinoma cells (BT-474)

Biophys Chem. 2023 Sep:300:107077. doi: 10.1016/j.bpc.2023.107077. Epub 2023 Jul 19.

Authors

M B Kobal¹, S A Camacho², L G Moreira¹, K A Toledo³, D B Tada⁴, P H B Aoki⁵

Affiliations

¹ São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP 19806-900, Brazil.
² São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP 19806-900, Brazil; University of São Paulo (USP), São Carlos Institute of Physics (IFSC), São Carlos, SP 13566-590, Brazil.
³ São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP 19806-900, Brazil; São Paulo State University (UNESP), Institute of Biosciences, Letters and Exact Sciences, São José do Rio Preto, SP 15054-000, Brazil.
⁴ Federal University of São Paulo (UNIFESP), Institute of Science and Technology, São José dos Campos, SP 12231-280, Brazil.
⁵ São Paulo State University (UNESP), School of Sciences, Humanities and Languages, Assis, SP 19806-900, Brazil. Electronic address: pedro.aoki@unesp.br.

PMID: 37515949
DOI: 10.1016/j.bpc.2023.107077

Abstract

Gold nanoparticles are valuable photothermal agents owing to their efficient photothermal conversion, photobleaching resistance, and potential surface functionalization. Herein, we combined bioinspired membranes with in vitro assays to elicit the molecular mechanisms of gold shell-isolated nanoparticles (AuSHINs) on ductal mammary carcinoma cells (BT-474). Langmuir and Langmuir-Schaefer (LS) films were handled to build biomembranes from BT-474 lipid extract. AuSHINs incorporation led to surface pressure-area (π-A) isotherms expansion, increasing membrane flexibility. Fourier-transform infrared spectroscopy (FTIR) of LS multilayers revealed electrostatic AuSHINs interaction with head portions of BT-474 lipid extract, causing lipid chain disorganization. Limited AuSHINs insertion into monolayer contributed to hydroperoxidation of the unsaturated lipids upon irradiation, consistently with the surface area increments of ca. 2.0%. In fact, membrane disruption of irradiated BT-474 cells containing AuSHINs was confirmed by confocal microscopy and LDH leakage, with greater damage at 2.2 × 10¹³ AuSHINs/mL. Furthermore, the decrease in nuclei dimensions indicates cell death through photoinduced damage.

Keywords: BT-474 cell culture; Bioinspired tumoral membranes; Photothermal therapy; Plasmonic gold nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma*
Cell Line, Tumor
Gold / chemistry
Humans
Lipids
Metal Nanoparticles*
Nanoparticles* / chemistry

Substances

Gold
Lipids