The Additional 15 nt of 5' UTR in a Novel Recombinant Isolate of Chilli Veinal Mottle Virus in Solanum nigrum L. Is Crucial for Infection

Qionglian Wan; Kaiyue Zheng; Jian Wu; Shan Bu; Mengting Jiao; Huijie Zhou; Yuwen Lu; Hongying Zheng; Guanwei Wu; Shaofei Rao; Hairu Chen; Fei Yan; Jiejun Peng

doi:10.3390/v15071428

The Additional 15 nt of 5' UTR in a Novel Recombinant Isolate of Chilli Veinal Mottle Virus in Solanum nigrum L. Is Crucial for Infection

Viruses. 2023 Jun 23;15(7):1428. doi: 10.3390/v15071428.

Authors

Qionglian Wan^{1

2

3

4}, Kaiyue Zheng^{2

3}, Jian Wu^{2

3}, Shan Bu^{2

3}, Mengting Jiao^{2

3}, Huijie Zhou^{2

3}, Yuwen Lu^{2

3}, Hongying Zheng^{2

3}, Guanwei Wu^{2

3}, Shaofei Rao^{2

3}, Hairu Chen¹, Fei Yan^{2

3}, Jiejun Peng^{2

3}

Affiliations

¹ College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
³ Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
⁴ School of Chemistry, Biology and Environment, Yuxi Normal University, Yuxi 653100, China.

Abstract

An isolate of chilli veinal mottle virus (ChiVMV; genus Potyvirus) of Solanum nigrum L. from southwest China (ChiVMV-YunN/Yuxi) was identified and sequenced (GenBank: OP404087). Comparison with other ChiVMV isolates and recombination analyses suggested a recombinant origin. The most significant recombination event among all 21 complete ChiVMV isolates was an ending breakpoint at 1408-1488 for ChiVMV-YunN/Yuxi with ChiVMV-TaiW and ChiVMV-YunN/Ca operating as the respective major and minor parents. Interestingly, the 5' UTR of ChiVMV-YunN/Yuxi is 15 nucleotides ('AAAAATAAAACAACC') longer than other reported isolates. A full-length clone of ChiVMV-YunN/Yuxi was constructed and was shown to be infectious in Nicotiana benthamiana. The additional 15 nt of 5' UTR in ChiVMV-YunN/Yuxi was stable when transmitted through three generations. Experiments with modified clones showed that the additional 15 nt are essential for infection by this isolate.

Keywords: chilli veinal mottle virus; genetic diversity; infectious clone; potyvirus; recombination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

5' Untranslated Regions
China
Plant Diseases
Potyvirus*
Solanum nigrum*

Substances

5' Untranslated Regions

Grants and funding

This work was supported by National Key R&D Program of China (2022YFD1401200), Chinese Agriculture Research System of MOF and MARA (CARS-24-C-04) and K. C. Wong education foundation.