Preparation of Selenium-Based Drug-Modified Polymeric Ligand-Functionalised Fe3O4 Nanoparticles as Multimodal Drug Carrier and Magnetic Hyperthermia Inductor

Itziar Galarreta-Rodriguez; Mikel Etxebeste-Mitxeltorena; Esther Moreno; Daniel Plano; Carmen Sanmartín; Saad Megahed; Neus Feliu; Wolfgang J Parak; Eneko Garaio; Izaskun Gil de Muro; Luis Lezama; Idoia Ruiz de Larramendi; Maite Insausti

doi:10.3390/ph16070949

Preparation of Selenium-Based Drug-Modified Polymeric Ligand-Functionalised Fe₃O₄ Nanoparticles as Multimodal Drug Carrier and Magnetic Hyperthermia Inductor

Pharmaceuticals (Basel). 2023 Jun 30;16(7):949. doi: 10.3390/ph16070949.

Authors

Itziar Galarreta-Rodriguez¹, Mikel Etxebeste-Mitxeltorena^{2

3}, Esther Moreno^{4

5

6}, Daniel Plano^{2

3}, Carmen Sanmartín^{2

3}, Saad Megahed^{5

6}, Neus Feliu⁷, Wolfgang J Parak⁵, Eneko Garaio^{8

9}, Izaskun Gil de Muro^{1

10}, Luis Lezama¹, Idoia Ruiz de Larramendi¹, Maite Insausti^{1

10}

Affiliations

¹ Departamento Química Orgánica e Inorgánica, Facultad de Ciencia y Tecnología, University of the Basque Country (UPV/EHU), Sarriena s/n, 48940 Leioa, Spain.
² Department of Pharmaceutical Technology and Chemistry, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain.
³ The Navarra Medical Research Institute (IdiSNA), Irunlarrea 3, 31008 Pamplona, Spain.
⁴ Tropical Health Institute of the University of Navarra (ISTUN), University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain.
⁵ Fachbereich Physik, Universität Hamburg, 22761 Hamburg, Germany.
⁶ Physics Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
⁷ Center for Applied Nanotechnology CAN, Fraunhofer Institute for Applied Polymer Research IAP, 20146 Hamburg, Germany.
⁸ Departamento de Ciencias, Universidad Pública de Navarra, Campus Arrosadía, 31006 Pamplona, Spain.
⁹ Institute for Advanced Materials and Mathematics (INAMAT2), Universidad Pública de Navarra, Campus de Arrosadía, 31006 Pamplona, Spain.
¹⁰ BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940 Leioa, Spain.

Abstract

In recent years, much effort has been invested into developing multifunctional drug delivery systems to overcome the drawbacks of conventional carriers. Magnetic nanoparticles are not generally used as carriers but can be functionalised with several different biomolecules and their size can be tailored to present a hyperthermia response, allowing for the design of multifunctional systems which can be active in therapies. In this work, we have designed a drug carrier nanosystem based on Fe₃O₄ nanoparticles with large heating power and 4-amino-2-pentylselenoquinazoline as an attached drug that exhibits oxidative properties and high selectivity against a variety of cancer malignant cells. For this propose, two samples composed of homogeneous Fe₃O₄ nanoparticles (NPs) with different sizes, shapes, and magnetic properties have been synthesised and characterised. The surface modification of the prepared Fe₃O₄ nanoparticles has been developed using copolymers composed of poly(ethylene-alt-maleic anhydride), dodecylamine, polyethylene glycol and the drug 4-amino-2-pentylselenoquinazoline. The obtained nanosystems were properly characterised. Their in vitro efficacy in colon cancer cells and as magnetic hyperthermia inductors was analysed, thereby leaving the door open for their potential application as multimodal agents.

Keywords: drug carriers; magnetic hyperthermia inductor; magnetic nanoparticles.

Abstract

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