Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)

Xia Zhu; Yongtao Liu; Ning Xu; Xiaohui Ai; Yibin Yang

doi:10.3390/pathogens12070886

Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)

Pathogens. 2023 Jun 29;12(7):886. doi: 10.3390/pathogens12070886.

Authors

Xia Zhu¹, Yongtao Liu¹, Ning Xu¹, Xiaohui Ai¹, Yibin Yang¹

Affiliation

¹ Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.

Abstract

IL-10 and IL-6 play important roles in protecting against inflammation and clearing pathogens from the body. In this study, homologous compounds of IL-10 and IL-6 were identified in channel catfish, and their immune responses were analyzed. The CDS sequences of IL-10 and IL-6 were 549 bp and 642 bp, respectively, and showed the highest homology with Ameiurus melas. In addition, the expression of the IL-10 and IL-6 genes was ubiquitous in 10 tissues examined. IL-10 is highly expressed in the liver and slightly expressed in the gill. The high expression of the IL-6 gene was observed in the spleen, heart, and gonad, with the lowest levels in the liver. LPS, Poly(I:C), PHA, and PMA showed a highly significant increase in IL-10 and IL-6 expression 48 h after CCK stimulation (p < 0.01). Otherwise, Yersinia ruckeri, Streptococcus iniae, channel catfish virus, and deltamethrin induced IL-10 and IL-6 expression, varying in intensity between different organs. Our results suggest that IL-10 and IL-6 are involved in the immune response of the host against the pathogen.

Keywords: channel catfish; immune response; interleukin-10; interleukin-6.

Grants and funding

This work was supported by the Natural Science Foundation of Hubei Province (No. 2022CFB631), the Central Public-interest Scientific Institution Basal Research Fund (No. YFI202209), and the Yancheng Fishery High Quality Development Project (No. YCSCYJ2021026).