Selection and Validation of Reference Genes in Virus-Infected Sweet Potato Plants

Guangyan Li; Xiaohui Sun; Xiaoping Zhu; Bin Wu; Hao Hong; Zhimei Xin; Xiangqi Xin; Jiejun Peng; Shanshan Jiang

doi:10.3390/genes14071477

Selection and Validation of Reference Genes in Virus-Infected Sweet Potato Plants

Genes (Basel). 2023 Jul 19;14(7):1477. doi: 10.3390/genes14071477.

Authors

Guangyan Li¹, Xiaohui Sun¹, Xiaoping Zhu², Bin Wu¹, Hao Hong¹, Zhimei Xin¹, Xiangqi Xin¹, Jiejun Peng³, Shanshan Jiang^{1

3}

Affiliations

¹ Shandong Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
² Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests, College of Plant Protection, Shandong Agricultural University, Taian 271018, China.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.

Abstract

Quantitative real-time PCR (qRT-PCR) in sweet potatoes requires accurate data normalization; however, there are insufficient studies on appropriate reference genes for gene expression analysis. We examined variations in the expression of eight candidate reference genes in the leaf and root tissues of sweet potatoes (eight nonvirus-infected or eight virus-infected samples). Parallel analyses with geNorm, NormFinder, and Best-Keeper show that different viral infections and origin tissues affect the expression levels of these genes. Based on the results of the evaluation of the three software, the adenosine diphosphate-ribosylation factor is suitable for nonvirus or virus-infected sweet potato leaves. Cyclophilin and ubiquitin extension proteins are suitable for nonvirus-infected sweet potato leaves. Phospholipase D1 alpha is suitable for virus-infected sweet potato leaves. Actin is suitable for roots of nonvirus-infected sweet potatoes. Glyceraldehyde-3-phosphate dehydrogenase is suitable for virus-infected sweet potato roots. The research provides appropriate reference genes for further analysis in leaf and root samples of viruses in sweet potatoes.

Keywords: experimental conditions; expression stability; quantitative real-time PCR; reference genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling
Genes, Plant
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
Ipomoea batatas* / genetics
Plant Viruses* / genetics
Real-Time Polymerase Chain Reaction

Substances

Glyceraldehyde-3-Phosphate Dehydrogenases

Grants and funding

This research was funded by the Natural Science Foundation of Zhejiang Province (LY22C140001), the National Key R&D Program of China (2022YFD1401200), and the Agricultural Scientific and Technological Innovation Project at the Shandong Academy of Agricultural Sciences (CXGC2023A24).