Mn-XRN1 Has an Inhibitory Effect on Ovarian Reproduction in Macrobrachium nipponense

Tianyong Chen; Huwei Yuan; Hui Qiao; Sufei Jiang; Wenyi Zhang; Yiwei Xiong; Hongtuo Fu; Shubo Jin

doi:10.3390/genes14071454

Mn-XRN1 Has an Inhibitory Effect on Ovarian Reproduction in Macrobrachium nipponense

Genes (Basel). 2023 Jul 16;14(7):1454. doi: 10.3390/genes14071454.

Authors

Tianyong Chen¹, Huwei Yuan², Hui Qiao³, Sufei Jiang³, Wenyi Zhang³, Yiwei Xiong³, Hongtuo Fu^{1

3}, Shubo Jin³

Affiliations

¹ National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
² Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
³ Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.

Abstract

XRN1 is an exoribonuclease that degrades mRNA in the cytoplasm along the 5'-3' direction. A previous study indicated that it may be involved in the reproduction of Macrobrachium nipponense. Quantitative real-time PCR was used to detect the spatiotemporal expression pattern of Mn-XRN1. At the tissue level, Mn-XRN1 was significantly expressed in the ovary. During development, Mn-XRN1 was significantly expressed at the CS stage of the embryo, on the 10th day post-larval and in the O2 stage of ovarian reproduction. The in situ hybridization results showed the location of Mn-XRN1 in the ovary. The expression of Mn-VASA was significantly increased after in vivo injection of Mn-XRN1 dsRNA. This suggests that Mn-XRN1 negatively regulates the expression of Mn-VASA. Furthermore, we counted the number of M. nipponense at various stages of ovarian reproduction on different days after RNAi. The results showed that ovarian development was significantly accelerated. In general, the results of the present study indicate that Mn-XRN1 has an inhibitory effect on the ovarian maturation of M. nipponense. The inhibitory effect might be through negative regulation of Mn-VASA.

Keywords: Macrobrachium nipponense; VASA; XRN1; metamorphosis; ovarian reproduction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Female
Ovary / metabolism
Palaemonidae* / genetics
Palaemonidae* / metabolism
RNA Interference

Grants and funding

This research was supported by grants from Central Public-Interest Scientific Institution Basal Research Fund (NO.2023JBFM04, 2020TD36); Seed Industry Revitalization Project of Jiangsu Province (JBGS (2021) 118); Jiangsu Agricultural Industry Technology System; the earmarked fund for CARS-48; the New cultivar breeding Major Project of Jiangsu province (PZCZ201745); the Natural Science Foundation of Jiangsu Province (BK20221207). Thanks for the Jiangsu Province Platform for the Conservation and Utilization of Agricultural Germplasm.