Evaluation the in vivo behaviors of PM2.5 in rats using noninvasive PET imaging with mimic particles

Donghui Pan; Yuping Xu; Xinyu Wang; Lizhen Wang; Junjie Yan; Dongjian Shi; Min Yang; Mingqing Chen

doi:10.1016/j.chemosphere.2023.139663

Evaluation the in vivo behaviors of PM_2.5 in rats using noninvasive PET imaging with mimic particles

Chemosphere. 2023 Oct:339:139663. doi: 10.1016/j.chemosphere.2023.139663. Epub 2023 Jul 26.

Authors

Donghui Pan¹, Yuping Xu², Xinyu Wang², Lizhen Wang², Junjie Yan², Dongjian Shi³, Min Yang⁴, Mingqing Chen⁵

Affiliations

¹ Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China; Key Laboratory of Nuclear Medicine, National Health Commission, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, China.
² Key Laboratory of Nuclear Medicine, National Health Commission, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, China.
³ Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China.
⁴ Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China; Key Laboratory of Nuclear Medicine, National Health Commission, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, Wuxi, 214063, China. Electronic address: yangmin@jsinm.org.
⁵ Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, 214122, China. Electronic address: mqchen@jiangnan.edu.cn.

PMID: 37506893
DOI: 10.1016/j.chemosphere.2023.139663

Abstract

Inhaled PM_2.5 particles is harmful to human health. However, real-time tracking of PM_2.5 particles and dynamic evaluation of the pharmacokinetic behaviors in vivo are still challenging. Here, PET imaging is utilized to noninvasively monitor the in vivo behavior of PM_2.5 particles in rats. To mimic aerosol PM_2.5 particles suspended in ambient air, ⁸⁹Zr-labeled melanin nanoparticles (⁸⁹Zr-MNP) are nebulized into microscopic liquid particles with a mean size of 2.5 μm. Then, the ⁸⁹Zr-labeled PM_2.5 mimic particles (⁸⁹Zr-PM_2.5) are administrated into rats via inhalation. PET imaging showed that ⁸⁹Zr-PM_2.5 mainly accumulated in the lungs for up to 384 h after administration. Besides, we also observe that a small amount of ⁸⁹Zr-PM_2.5 can penetrate the brain through the inhalation. Further PET imaging showed that enhanced uptakes of ¹⁸F-FDG and ¹⁸F-DPA-714 were found in the brain of rats upon PM_2.5 mimic particle exposure, which revealed that pulmonary exposure to PM_2.5 could cause potential damages to the brain. Note that abnormal glucose metabolism was reversed, but the neuroinflammation was permanent and could not be alleviated after ceasing PM_2.5 exposure. Our results demonstrate that PET is a sensitive and feasible tool for evaluating the in vivo behaviors of PM_2.5.

Keywords: (89)Zr labeling; In vivo tracking; Melanin nanoparticles; PET imaging; PM(2.5).

MeSH terms

Animals
Brain / diagnostic imaging
Humans
Lung* / diagnostic imaging
Particulate Matter / toxicity
Positron-Emission Tomography*
Rats

Substances

Particulate Matter