A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4'-Dinitrocarbanilide in Chicken

Qianxin Liang; Chen Chen; Wenqing Xu; Ning Zhang; Jielin Yang; Wei Song; Huimei Cai; Ruyan Hou; Hongfang Li; Xiya Zhang

doi:10.3390/toxics11070628

A Comparable icELISA and Lateral Flow Immunoassay for the Sensitive and Rapid Detection of 4,4'-Dinitrocarbanilide in Chicken

Toxics. 2023 Jul 20;11(7):628. doi: 10.3390/toxics11070628.

Authors

Qianxin Liang¹, Chen Chen¹, Wenqing Xu¹, Ning Zhang¹, Jielin Yang¹, Wei Song², Huimei Cai¹, Ruyan Hou¹, Hongfang Li¹, Xiya Zhang³

Affiliations

¹ Animal-Derived Food Safety Innovation Team, College of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, China.
² Hefei Customs District Technical Center, Anhui Key Lab of Analysis and Detection for Food Safety, Hefei 230022, China.
³ College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China.

Abstract

4,4'-dinitrocarbanilide (DNC) is a key component and marker residue of nicarbazin, which forms residues in edible tissue and then causes nephrotoxicity and hepatotoxicity in humans if used excessively. To simplify sample preparation and monitor the DNC rapidly and accurately, a comparable icELISA and lateral flow immunoassay (LFIA) was developed in this study. Briefly, the reaction parameters were explored for improving the sensitivity of icELISA and LFIA. Under the optimal conditions, methanol was selected as the extracting solvent for DNC in chicken, and 20- and 10-fold dilutions of sample extraction eliminated the matrix effect for icELISA and LFIA, separately. After sample pretreatment, the analysis properties of icELISA and LFIA were compared. The limit of detection of icELISA for DNC was 0.8 μg/kg, and the visual and quantitative limits of detection of LFIA were 8 and 2.5 μg/kg. Compared with icELISA, LFIA showed lower sensitivity but obvious advantages in terms of matrix tolerance and detection time (within 15 min). The sensitivity, specificity, and accuracy of the developed assays satisfied the detection requirement even if using simple sample pretreatment. This comparable icELISA and LFIA provided mutual verifiability methods for the accurate detection of DNC in chicken.

Keywords: 4,4′-dinitrocarbanilide; chicken; icELISA; lateral flow immunoassay; rapid detection.

Grants and funding

This work was supported by the Natural Science Foundation of Anhui Province (Grant No. 2108085QC140), Natural Science Foundation of the Education Department of Anhui Province (Gant No. 2022AH050891), Project of Animal-derived Food Safety Innovation Team (Gant No. ANRC2021040), Introduction and Stabilization Project of Talent Project of Anhui Agricultural University (Grant No. rc352003), Key Generic Technology Research and Development Program of Hefei City (Grant No. 2021GJ081) and Scientific Research Project of General Administration of Customs (Grant No. 2021HK196).