The Fabrication of a La2Sn2O7/ f-HNT Composite for Non-Enzymatic Electrochemical Detection of 3-Nitro-l-tyrosine in Biological Samples

Balasubramanian Sriram; Sakthivel Kogularasu; Sea-Fue Wang; Guo-Ping Chang-Chien

doi:10.3390/bios13070722

The Fabrication of a La₂Sn₂O₇/ f-HNT Composite for Non-Enzymatic Electrochemical Detection of 3-Nitro-l-tyrosine in Biological Samples

Biosensors (Basel). 2023 Jul 10;13(7):722. doi: 10.3390/bios13070722.

Authors

Balasubramanian Sriram¹, Sakthivel Kogularasu², Sea-Fue Wang¹, Guo-Ping Chang-Chien^{3

4

5}

Affiliations

¹ Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, Taipei 106, Taiwan.
² Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
³ Institute of Environmental Toxin and Emerging-Contaminant Research, Cheng Shiu University, Kaohsiung 833301, Taiwan.
⁴ Super Micro Mass Research and Technology Center, Cheng Shiu University, Kaohsiung 833301, Taiwan.
⁵ Center for Environmental Toxin and Emerging-Contaminant Research, Cheng Shiu University, Kaohsiung 833301, Taiwan.

Abstract

Reactive oxygen and nitrogen species (RONS), including 3-nitro-l-tyrosine, play a dual role in human health, inducing oxidative damage and regulating cellular functions. Early and accurate detection of such molecules, such as L-tyrosine in urine, can serve as critical biomarkers for various cancers. In this study, we aimed to enhance the electrochemical detection of these molecules through the synthesis of La₂Sn₂O₇/f-HNT nanocomposites via a simple hydrothermal method. Detailed structural and morphological characterizations confirmed successful synthesis, consistent with our expected outcomes. The synthesized nanocomposites were utilized as nanocatalysts in electrochemical sensors, showing a notable limit of the detection of 0.012 µM for the real-time detection of 3-nitro-l-tyrosine. These findings underscore the potential of nanomaterial-based sensors in advancing early disease detection with high sensitivity, furthering our understanding of cellular oxidative processes.

Keywords: 3-nitro-l-tyrosine; La2Sn2O7; electrochemical biosensor; f-HNT; real-time sensing.

MeSH terms

Electrochemical Techniques* / methods
Electrodes
Humans
Nanocomposites*
Reactive Oxygen Species
Tyrosine

Substances

3-nitrotyrosine
Tyrosine
Reactive Oxygen Species

Grants and funding

This research was funded by Cheng Shiu University-(MOST 111-2637-E-230-001), the National Cheng Kung University, and the National Taipei University of Technology.