Bone morphogenic protein-7 (BMP-7) polymorphism: Susceptibility to cirrhosis and hepatocellular carcinoma after viral hepatitis in Egyptian patients

Hind S AboShabaan; Osama Alghannam; Faisal Ismail; Islam M El-Garawani; Mohamed El-Shahat; Roba M Talaat; Eman A El-Maadawy; Nasser Hussein; Zeinab A Kasemy; Eman Abdelsameea; Soghra Haq; Heba M Hathout

doi:10.5114/ceh.2023.128616

Bone morphogenic protein-7 (BMP-7) polymorphism: Susceptibility to cirrhosis and hepatocellular carcinoma after viral hepatitis in Egyptian patients

Clin Exp Hepatol. 2023 Jun;9(2):154-163. doi: 10.5114/ceh.2023.128616. Epub 2023 Jun 30.

Authors

Hind S AboShabaan¹, Osama Alghannam², Faisal Ismail^{3

4

5}, Islam M El-Garawani⁶, Mohamed El-Shahat⁷, Roba M Talaat⁷, Eman A El-Maadawy⁷, Nasser Hussein⁷, Zeinab A Kasemy⁸, Eman Abdelsameea⁹, Soghra Haq³, Heba M Hathout¹⁰

Affiliations

¹ Department of Clinical Pathology, National Liver Institute, Menoufia University, Shebin El-Kom, Egypt.
² Department of Chemistry, Faculty of Science, Menoufia University, Menoufia, Egypt.
³ Department of Clinical Laboratory, Faculty of Medical Technology, University of Tobruk, Libya.
⁴ Department of Blood Transmitted Diseases, National Centre for Disease Control, Tobruk, Libya.
⁵ Department of Infectious Diseases, Libyan Medical Research Centre, Kambut, Tobruk, Libya.
⁶ Department of Zoology, Faculty of Science, Menoufia University, Menoufia, Egypt.
⁷ Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt.
⁸ Department of Public Health and Community Medicine, Faculty of Medicine, Menoufia University, Menoufia, Egypt.
⁹ Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Shebin El-Kom, Egypt.
¹⁰ Department of Natural Resources, Faculty of African Postgraduate Studies, Cairo University, Giza, Egypt.

Abstract

Aim of the study: Bone morphogenic proteins (BMPs) have both inhibitory and stimulatory effects on growth of a tumor that depend on the type of cells, the dosage and the tumor microenvironment. We aimed to investigate the impact of the bone morphogenic protein-7 (BMP-7) single nucleotide polymorphism (SNP) rs230205 [A/G] on susceptibility to hepatocellular carcinoma (HCC) progression from liver cirrhosis after viral hepatitis infection in Egyptian patients.

Material and methods: The amplification-refractory mutation system (ARMS)-polymerase chain reaction (PCR) method was used to genotype the rs230205 [A/G] SNP in 150 subjects (50 patients with post-hepatitis C or B cirrhosis, 50 HCC patients, and 50 controls). Expression level of BMP-7 protein was assessed using enzyme-linked immunosorbent assay (ELISA).

Results: The results revealed insignificant changes in distribution of all genotypes/alleles of the BMP-7 rs230205 [A/G] SNP between cirrhotic patients, HCC patients and controls. The AA genotype and A allele could be considered risk factors for cirrhosis (OR = 1.75, 1.50) and HCC (OR = 2.19, 1.74), respectively. The AA genotype (95% CI: 0.45-6.79) and A allele (OR = 1.50, 95% CI: 0.77-2.93) may be viewed as cirrhosis risk factors based on group segregation. Additionally, the A allele, AG and AA genotypes and their combined ORs of 2.19 (95% CI: 0.58-8.23), 1.74 (95% CI: 0.90-3.37), and 1.70 (95% CI: 0.68-4.29) could all be risk factors for HCC. No genotype or allele could be regarded as a risk factor for progression of cirrhosis to HCC, according to OR values.

Conclusions: The results showed no correlation between BMP-7 rs230205 [A/G] SNP and progression of cirrhosis to HCC. To confirm our findings, additional prospective large-scale research is required.

Keywords: bone morphogenic protein (BMP)-7; hepatocellular carcinoma (HCC); liver cirrhosis; single nucleotide polymorphism (SNP).