Production of polyhydroxyalkanoates from hydrolysed rapeseed meal by Haloferax mediterranei

Thammarit Khamplod; Phavit Wongsirichot; James Winterburn

doi:10.1016/j.biortech.2023.129541

Production of polyhydroxyalkanoates from hydrolysed rapeseed meal by Haloferax mediterranei

Bioresour Technol. 2023 Oct:386:129541. doi: 10.1016/j.biortech.2023.129541. Epub 2023 Jul 25.

Authors

Thammarit Khamplod¹, Phavit Wongsirichot², James Winterburn³

Affiliations

¹ Department of Chemical Engineering, School of Engineering, Faculty of Science and Engineering, Engineering Building A, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom. Electronic address: k.thammarit1@gmail.com.
² Department of Chemical Engineering, School of Engineering, Faculty of Science and Engineering, Engineering Building A, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom. Electronic address: Phavit.wongsirichot@manchester.ac.uk.
³ Department of Chemical Engineering, School of Engineering, Faculty of Science and Engineering, Engineering Building A, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom. Electronic address: james.winterburn@manchester.ac.uk.

PMID: 37499923
DOI: 10.1016/j.biortech.2023.129541

Abstract

Rapeseed meal (RSM) hydrolysate is a potential low-cost feedstock for the production of polyhydroxyalkanoates (PHAs) by the archaea, Haloferax mediterranei. Acidic and enzymatic hydrolysis were carried out to compare effectiveness. Enzymatic hydrolysis is more effective than acidic hydrolysis for fermentation substrate leading to increased PHA productivity. H. mediterranei didn't grow or produce PHA when acid hydrolysed RSM medium was present in proportions greater than 25% (vol.), potentially due to the effect of inhibitors such as furfural, hydroxymethylfurfural (HMF), etc. However, H. mediterranei was able to grow and produce PHA when using enzymatically hydrolysed RSM medium. The maximum PHA concentration of 0.512 g/L was found at 75% (vol.) in enzymatic RSM hydrolysate medium. The biopolymer obtained had improved thermal and mechanical properties compared to PHB homopolymer. RSM's potential as a low-cost alternative feedstock for improved PHA production under non-sterile conditions was successfully demonstrated, and its usage should be further explored.

Keywords: Fermentation; Haloferax mediterranei; Hydrolysis; Polyhydroxyalkanoates; Rapeseed meal.

MeSH terms

Brassica napus* / metabolism
Brassica rapa* / metabolism
Fermentation
Haloferax mediterranei* / metabolism
Hydrolysis
Polyhydroxyalkanoates* / metabolism

Substances

Polyhydroxyalkanoates