Smartphone-based pH responsive 3-channel colorimetric biosensor for non-enzymatic multi-antibiotic residues

Xiaotian Zhou; Siyuan Fu; Jiahua Li; Yuhan Yi; Yaqi Hu; Ji Lu; Chao Yang; Jinfeng Miao; Yuanyuan Xu

doi:10.1016/j.foodchem.2023.136953

Smartphone-based pH responsive 3-channel colorimetric biosensor for non-enzymatic multi-antibiotic residues

Food Chem. 2023 Dec 15:429:136953. doi: 10.1016/j.foodchem.2023.136953. Epub 2023 Jul 21.

Authors

Xiaotian Zhou¹, Siyuan Fu¹, Jiahua Li¹, Yuhan Yi¹, Yaqi Hu¹, Ji Lu¹, Chao Yang², Jinfeng Miao³, Yuanyuan Xu⁴

Affiliations

¹ Sanya Institute of Nanjing Agricultural University, MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
² Xidian University Hangzhou Institute of Technology, Hangzhou 311231, China.
³ Sanya Institute of Nanjing Agricultural University, MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: miaojinfeng@njau.edu.cn.
⁴ Sanya Institute of Nanjing Agricultural University, MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: xuyuanyuan@njau.edu.cn.

PMID: 37499511
DOI: 10.1016/j.foodchem.2023.136953

Abstract

Antibiotic residues in animal-derived food pose a risk to food safety and human health. Here, a smartphone-based pH-responsive 3-channel colorimetric biosensor is constructed for rapid detection of non-enzymatic multi-antibiotic residues in milk. In this system, a magnetic separation and enrichment approach is designed to specifically capture different antibiotic residues in complex environment. Indicators loaded on polydopamine-silver nanoparticles with excellently pH responsive visualization properties are utilized to ensure the high sensitivity of detection system. Moreover, smartphones are introduced to fulfill the demand for portable and on-site inspection of practical applications. It achieves simultaneous detection of oxytetracycline, kanamycin and streptomycin in the linear range of 1-10⁵ pg/mL with detection limits of 0.085, 0.168, and 0.307 pg/mL, respectively. The practicality of the reported multi-antibiotic residues detection system is successfully demonstrated and evaluated challenging milk samples. Therefore, this system demonstrates the wide applications in multi-antibiotic residue analysis and food safety guarantee.

Keywords: Antibiotic residues; Multiple colorimetric channels; On-site detection; Smartphones; pH responsive nanoparticles.

MeSH terms

Animals
Anti-Bacterial Agents / analysis
Biosensing Techniques*
Colorimetry
Humans
Hydrogen-Ion Concentration
Limit of Detection
Metal Nanoparticles* / chemistry
Silver / chemistry
Smartphone

Substances

Anti-Bacterial Agents
Silver