DNA intercalation makes possible superior-gain organic photoelectrochemical transistor detection

Peng Ju; Yu-Yue Zhu; Tian-Tong Jiang; Ge Gao; Shi-Liang Wang; Xing-Wu Jiang; Yi-Tong Xu; Xiao-Fan Zhai; Hong Zhou; Wei-Wei Zhao

doi:10.1016/j.bios.2023.115543

DNA intercalation makes possible superior-gain organic photoelectrochemical transistor detection

Biosens Bioelectron. 2023 Oct 1:237:115543. doi: 10.1016/j.bios.2023.115543. Epub 2023 Jul 22.

Authors

Peng Ju¹, Yu-Yue Zhu², Tian-Tong Jiang³, Ge Gao⁴, Shi-Liang Wang⁵, Xing-Wu Jiang⁶, Yi-Tong Xu⁷, Xiao-Fan Zhai⁸, Hong Zhou⁹, Wei-Wei Zhao¹⁰

Affiliations

¹ Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation, Qingdao, 266061, China; College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, PR China.
² Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; College of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.
³ Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China.
⁴ State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
⁵ Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao, 266590, PR China.
⁶ Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, No. 6 Xianxialing Road, Qingdao, 266061, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; Shandong Key Laboratory of Marine Ecological Environment and Disaster Prevention and Mitigation, Qingdao, 266061, China.
⁷ State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
⁸ CAS Key Laboratory of Marine Environmental Corrosion and Bio-fouling, Institute of Oceanology, Chinese Academy of Sciences, No. 7 Nanhai Road, Qingdao, 266071, China. Electronic address: zhaixf@qdio.ac.cn.
⁹ Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address: zhouhong@qust.edu.cn.
¹⁰ State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China. Electronic address: zww@nju.edu.cn.

PMID: 37499378
DOI: 10.1016/j.bios.2023.115543

Abstract

DNA intercalation has increasingly been studied for various scenario implementations due to the diverse functions of DNA/intercalators. Nascent organic photoelectrochemical transistor (OPECT) biosensing taking place in organic electronics and photoelectrochemical bioanalysis represents a promising technological frontier in the arena. In this work, we first devise DNA intercalation-enabled OPECT for miRNA detection with a superior gain up to 17100. Intercalation of [Ru(bpy)₂dppz]²⁺ within the miRNA-initiated hybrid chain reaction (HCR)-derived duplex DNA is realized for producing anodic photocurrent upon light stimulation, causing the corresponding target-dependent alternation in gate voltage (V_G) and hence the modulated channel current (I_DS) of poly (3,4-ethylenedioxythiophene) doped with poly (styrene sulfonate) (PEDOT:PSS) under specific drain voltage (V_DS) for quantitative miRNA-21 analysis, which shows a wide linear relationship and a low detection limit of 5.5 × 10^-15 mol L^-1. This study features the DNA intercalation-enabled organic electronics with superior gain and is envisaged to attract more attention to explore DNA adducts for innovative bioelectronics and biosensing, given the diverse DNA binders with multiple functions.

Keywords: Bioanalysis; DNA intercalation; Organic photoelectrochemical electronics; Superior gain.

MeSH terms

Biosensing Techniques*
DNA / analysis
Intercalating Agents
MicroRNAs*
Styrene

Substances

DNA
MicroRNAs
Styrene
Intercalating Agents