QHRD106 ameliorates ischemic stroke injury as a long-acting tissue kallikrein preparation

Si-Yi Xu; Jun-Qiu Jia; Min Sun; Xin-Yu Bao; Sheng-Nan Xia; Shu Shu; Pin-Yi Liu; Sen-Lin Ji; Lei Ye; Xiang Cao; Yun Xu

doi:10.1016/j.isci.2023.107268

QHRD106 ameliorates ischemic stroke injury as a long-acting tissue kallikrein preparation

iScience. 2023 Jul 3;26(7):107268. doi: 10.1016/j.isci.2023.107268. eCollection 2023 Jul 21.

Authors

Si-Yi Xu^{1

2}, Jun-Qiu Jia², Min Sun², Xin-Yu Bao^{2

3

4

5}, Sheng-Nan Xia^{2

3

4

5}, Shu Shu^{2

3

4

5}, Pin-Yi Liu^{2

3

4

5}, Sen-Lin Ji^{2

3

4

5}, Lei Ye², Xiang Cao^{1

2

3

4

5}, Yun Xu^{1

2

3

4

5}

Affiliations

¹ Department of Neurology, Nanjing Drum Tower Hospital Clinical College of Jiangsu University, Nanjing, Jiangsu 210008, P.R. China.
² Department of Neurology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School and State Key Laboratory of Pharmaceutical Biotechnology, Institute of Translational Medicine for Brain Critical Diseases, Nanjing University, Nanjing, Jiangsu 210008, P.R. China.
³ Jiangsu Key Laboratory for Molecular Medicine, Medical School of Nanjing University, Nanjing, Jiangsu 210008, P.R. China.
⁴ Jiangsu Provincial Key Discipline of Neurology, Nanjing, Jiangsu 210008, P.R. China.
⁵ Nanjing Neurology Medical Center, Nanjing, Jiangsu 210008, P.R. China.

Abstract

Ischemic stroke is the second leading cause of death worldwide, and there are limited effective treatment strategies. QHRD106, a polyethyleneglycol (PEG)-modified long-acting tissue kallikrein preparation, has not been reported previously. In this study, we aimed to investigate the therapeutic effect of QHRD106 in ischemic stroke and its possible mechanism. We found that QHRD106 treatment alleviated brain injury after stroke via bradykinin (BK) receptor B2 (B2R) instead of BK receptor B1 (B1R). Mechanistically, QHRD106 reduced high-mobility group box 1 (HMGB1)-induced apoptosis and inflammation after ischemic stroke in vivo and in vitro. Moreover, we confirmed that QHRD106 reduced the level of acetylated HMGB1 and reduced the binding between heat shock protein 90 alpha family class A member 1 (HSP90AA1) and HMGB1, thus inhibiting the translocation and release of HMGB1. In summary, these findings indicate that QHRD106 treatment has therapeutic potential for cerebral ischemic stroke.

Keywords: Biochemistry; Neuroscience; Pharmacology; Physiology.