Relation between interleukin-6 concentrations and oxidative status of HIV infected patients with /or at risk of Kaposi disease in Yaounde

Roger Ahouga Voufo; Armand Emmanuel Kouotou; Nchinda Jones Tatah; Georges TeTo; Cédric Gueguim; Chantal Marie Essome Ngondé; Armand Gabin Njiguet Tepa; Arnaud Gabin; Falmata Amazia; Natacha Lena Yembeau; Charles Kouanfack; Pieme Constant Anatole

doi:10.1186/s12985-023-02109-9

Relation between interleukin-6 concentrations and oxidative status of HIV infected patients with /or at risk of Kaposi disease in Yaounde

Virol J. 2023 Jul 25;20(1):165. doi: 10.1186/s12985-023-02109-9.

Affiliations

¹ Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaounde, Cameroon.
² Department of Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaounde, Cameroon.
³ Department of Internal Medicine Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaounde, Cameroon.
⁴ Centre International de Référence Chantal Biya (CICRB), Yaounde, Cameroon.
⁵ Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.
⁶ Faculty of Medicine and pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
⁷ Department of Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaounde, Cameroon. apieme@yahoo.fr.

Abstract

Objective: To investigate the relation between interleukin-6 concentration and oxidative status of HIV infected patients with or at risk of Kaposi's disease in Yaoundé.

Methods: We conducted a two-months cross-sectional study on 87 consenting HIV infected patients followed at the Day Hospital of the Yaoundé Central Hospital. Serum/plasma obtained after centrifugation of blood collected in dry/EDTA tubes was used for the determination of Human Herpes Virus-8 antigen (HHV-8) and Interleukin-6 (IL-6) by the ELISA technique, and that of oxidative stress markers: Malondialdehyde (MDA) reduced Glutathione (GSH) and total antioxidant capacity by spectrophotometry.

Results: Subjects belonging to the [40-50[year-old age group were mainly represented in our study population with 43.7%. The average age was 44.6 ± 10.4 years with extremes ranging from 26 to 72 years. The sex ratio was 0.24. Our population was mainly represented by people infected with HIV type I (90.8%) and 3.4% had developed clinical signs of Kaposi's disease. The prevalence of the HHV-8 antigen was 57.5%. Immune and oxidative parameters did not vary with age, sex and therapeutic line. We noted a significant increase in IL-6 concentrations in patients positive to the HHV-8 antigen for IL-6 concentrations < 37 (P = 0.005; CI= [0.40; 0.59]. MDA and GSH concentrations increased significantly with the HHV-8 infection (P < 0.0001; CI= [0.40; 0.59] and P < 0.0001; CI= [13.30;21.45], respectively). Total antioxidant capacity (FRAP) decreased significantly with HHV-8 infection (P = 0.004; CI= [-69.18; -13.78]). We noted a significant increase in MDA concentrations in patients taking their ARVs irregularly, (P < 0.0001).

Conclusion: Our study showed a weak positive correlation between IL-6 and MDA, a strong negative correlation between FRAP and MDA and a strong positive correlation between MDA and GSH highlighting the association of these few markers of oxidative stress and Il-6 to the risk of Kaposi's disease.

Keywords: HIV; Interleukin-6; Kaposi’s disease; Oxidative status.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antioxidants
Cameroon
Cross-Sectional Studies
HIV Infections* / complications
HIV-1*
Herpesviridae Infections*
Herpesvirus 8, Human*
Humans
Interleukin-6
Middle Aged
Sarcoma, Kaposi*
Xeroderma Pigmentosum*

Substances

Interleukin-6
Antioxidants