Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice

Yin Yang; Zhaoxi Yang; Maoxia Lv; Ang Jia; Junjun Li; Baitao Liao; Jing'an Chen; Zhengzheng Wu; Yi Shi; Yang Xia; Dezhong Yao; Ke Chen

doi:10.4103/1673-5374.375341

Morphological disruption and visual tuning alterations in the primary visual cortex in glaucoma (DBA/2J) mice

Neural Regen Res. 2024 Jan;19(1):220-225. doi: 10.4103/1673-5374.375341.

Authors

Yin Yang¹, Zhaoxi Yang¹, Maoxia Lv¹, Ang Jia², Junjun Li², Baitao Liao², Jing'an Chen³, Zhengzheng Wu¹, Yi Shi⁴, Yang Xia⁵, Dezhong Yao⁶, Ke Chen⁵

Affiliations

¹ Department of Ophthalmology, Sichuan Provincial People's Hospital, Medical School, University of Electronic Science and Technology of China, Chengdu, Sichuan Province, China.
² The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for NeuroInformation, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan Province, China.
³ Research Unit of NeuroInformation, Chinese Academy of Medical Sciences, Chengdu, Sichuan Province; Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China.
⁴ Health Management Center, Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China; Research Unit for Blindness Prevention of Chinese Academy of Medical Sciences (2019RU026), Sichuan Academy of Medical Sciences, Chengdu, Sichuan Province, China.
⁵ Department of Ophthalmology, Sichuan Provincial People's Hospital, Medical School; The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for NeuroInformation, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan Province, China.
⁶ Department of Ophthalmology, Sichuan Provincial People's Hospital, Medical School; The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Lab for NeuroInformation, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan Province; Research Unit of NeuroInformation, Chinese Academy of Medical Sciences, Chengdu, Sichuan Province, China; Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China; School of Electrical Engineering, Zhengzhou University, Zhengzhou, Henan Province, China.

Abstract

Glaucoma is a leading cause of irreversible blindness worldwide, and previous studies have shown that, in addition to affecting the eyes, it also causes abnormalities in the brain. However, it is not yet clear how the primary visual cortex (V1) is altered in glaucoma. This study used DBA/2J mice as a model for spontaneous secondary glaucoma. The aim of the study was to compare the electrophysiological and histomorphological characteristics of neurons in the V1 between 9-month-old DBA/2J mice and age-matched C57BL/6J mice. We conducted single-unit recordings in the V1 of light-anesthetized mice to measure the visually induced responses, including single-unit spiking and gamma band oscillations. The morphology of layer II/III neurons was determined by neuronal nuclear antigen staining and Nissl staining of brain tissue sections. Eighty-seven neurons from eight DBA/2J mice and eighty-one neurons from eight C57BL/6J mice were examined. Compared with the C57BL/6J group, V1 neurons in the DBA/2J group exhibited weaker visual tuning and impaired spatial summation. Moreover, fewer neurons were observed in the V1 of DBA/2J mice compared with C57BL/6J mice. These findings suggest that DBA/2J mice have fewer neurons in the V1 compared with C57BL/6J mice, and that these neurons have impaired visual tuning. Our findings provide a better understanding of the pathological changes that occur in V1 neuron function and morphology in the DBA/2J mouse model. This study might offer some innovative perspectives regarding the treatment of glaucoma.

Keywords: DBA/2J; degeneration; gamma band oscillations; glaucoma; primary visual cortex (V1); retina; single-unit recording; tuning curve.