Rapid colorimetric sensor for ultrasensitive and highly selective detection of Fumonisin B1 in cereal based on laccase-mimicking activity of silver phosphate nanoparticles

Xiaojuan Niu; Huanhuan He; Hang Ran; Zhen Wu; Yue Tang; Yuangen Wu

doi:10.1016/j.foodchem.2023.136903

Rapid colorimetric sensor for ultrasensitive and highly selective detection of Fumonisin B1 in cereal based on laccase-mimicking activity of silver phosphate nanoparticles

Food Chem. 2023 Dec 15:429:136903. doi: 10.1016/j.foodchem.2023.136903. Epub 2023 Jul 20.

Authors

Xiaojuan Niu¹, Huanhuan He², Hang Ran², Zhen Wu³, Yue Tang³, Yuangen Wu⁴

Affiliations

¹ Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China; College of Life Sciences, Guizhou Normal University, Guiyang 550001, China.
² Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China.
³ Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China.
⁴ Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China; Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China. Electronic address: ygwu@gzu.edu.cn.

PMID: 37487390
DOI: 10.1016/j.foodchem.2023.136903

Abstract

Ag₃PO₄ nanoparticles (NPs) was prepared through a facile coprecipitation method, and was first found to have excellent laccase-mimicking catalytic activity. The study confirms that Fumonisin B1 (FB1) can effectively hinder the production of superoxide anion (O₂^-) between Ag₃PO₄ NPs and dissolved oxygen, and further inhibit laccase-mimicking activity of Ag₃PO₄ NPs. Thus, a novel rapid colorimetric sensor for FB1 analysis in cereal was first established using laccase-mimicking activity as sensing signal. The absorbance variation of sensing solution is directly related to the amount of FB1, and the color change is further combined with smartphone for quantitively analysis of FB1. The limit of detection (LOD) of the sensor is determined as low as 1.73 μg·L^-1, which is far lower than the maximum residue limits (MRLs) of FB1 set by European Commission and US Food and Drug Administration (FDA). The average recovery of 87.8-104.5% for FB1 detection was obtained in cereal.

Keywords: Mycotoxins; Nanozyme; Oxygen radicals; RGB recognition; Visual detection.

MeSH terms

Colorimetry / methods
Edible Grain / chemistry
Fumonisins* / analysis
Laccase / analysis
Nanoparticles* / chemistry

Substances

fumonisin B1
silver phosphate
Laccase
Fumonisins