The beneficial effect of sulforaphane on platelet responsiveness during caloric load: a single-intake, double-blind, placebo-controlled, crossover trial in healthy participants

Hidde P van Steenwijk; Evi Winter; Edward Knaven; Jos F Brouwers; Myrthe van Baardwijk; Jasper B van Dalum; Teus J C Luijendijk; Frits H M van Osch; Freddy J Troost; Aalt Bast; Khrystyna O Semen; Alie de Boer

doi:10.3389/fnut.2023.1204561

The beneficial effect of sulforaphane on platelet responsiveness during caloric load: a single-intake, double-blind, placebo-controlled, crossover trial in healthy participants

Front Nutr. 2023 Jul 6:10:1204561. doi: 10.3389/fnut.2023.1204561. eCollection 2023.

Authors

Hidde P van Steenwijk¹, Evi Winter¹, Edward Knaven², Jos F Brouwers², Myrthe van Baardwijk^{3

4}, Jasper B van Dalum³, Teus J C Luijendijk⁵, Frits H M van Osch^{6

7}, Freddy J Troost^{8

9}, Aalt Bast^{10

11}, Khrystyna O Semen¹⁰, Alie de Boer¹

Affiliations

¹ Food Claims Centre Venlo, Faculty of Science and Engineering, Maastricht University, Maastricht, Netherlands.
² Research Group Analysis Techniques in the Life Sciences, Avans University of Applied Sciences, Breda, Netherlands.
³ Omnigen B.V., Delft, Netherlands.
⁴ Department of Pathology and Clinical Bioinformatics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Netherlands.
⁵ Stichting Control in Food and Flowers, Delfgauw, Netherlands.
⁶ Department of Clinical Epidemiology, VieCuri Medical Center, Venlo, Netherlands.
⁷ Department of Epidemiology, NUTRIM, Faculty of Health, Medicine and Life Sciences, Maastricht University, Maastricht, Netherlands.
⁸ Division of Gastroenterology-Hepatology, Department of Internal Medicine, School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Center+, Maastricht, Netherlands.
⁹ Food Innovation and Health, Centre for Healthy Eating and Food Innovation, Maastricht University, Maastricht, Netherlands.
¹⁰ University College Venlo, Faculty of Science and Engineering, Maastricht University, Maastricht, Netherlands.
¹¹ Department of Pharmacology and Toxicology, Faculty of Health, Medicine and Life Sciences, Maastricht University, Maastricht, Netherlands.

Abstract

Background and aims: As our understanding of platelet activation in response to infections and/or inflammatory conditions is growing, it is becoming clearer that safe, yet efficacious, platelet-targeted phytochemicals could improve public health beyond the field of cardiovascular diseases. The phytonutrient sulforaphane shows promise for clinical use due to its effect on inflammatory pathways, favorable pharmacokinetic profile, and high bioavailability. The potential of sulforaphane to improve platelet functionality in impaired metabolic processes has however hardly been studied in humans. This study investigated the effects of broccoli sprout consumption, as a source of sulforaphane, on urinary 11-dehydro-thromboxane B₂ (TXB₂), a stable thromboxane metabolite used to monitor eicosanoid biosynthesis and response to antithrombotic therapy, in healthy participants exposed to caloric overload.

Methods: In this double-blind, placebo-controlled, crossover trial 12 healthy participants were administered 16g of broccoli sprouts, or pea sprouts (placebo) followed by the standardized high-caloric drink PhenFlex given to challenge healthy homeostasis. Urine samples were collected during the study visits and analyzed for 11-dehydro-TXB₂, sulforaphane and its metabolites. Genotyping was performed using Illumina GSA v3.0 DTCBooster.

Results: Administration of broccoli sprouts before the caloric load reduced urinary 11-dehydro-TXB₂ levels by 50% (p = 0.018). The amount of sulforaphane excreted in the urine during the study visits correlated negatively with 11-dehydro-TXB₂ (r_s = -0.377, p = 0.025). Participants carrying the polymorphic variant NAD(P)H dehydrogenase quinone 1 (NQO1*2) showed decreased excretion of sulforaphane (p = 0.035).

Conclusion: Sulforaphane was shown to be effective in targeting platelet responsiveness after a single intake. Our results indicate an inverse causal relationship between sulforaphane and 11-dehydro-TXB₂, which is unaffected by the concomitant intake of the metabolic challenge. 11-Dehydro-TXB₂ shows promise as a non-invasive, sensitive, and suitable biomarker to investigate the effects of phytonutrients on platelet aggregation within hours.

Clinical trial registration: [https://clinicaltrials.gov/], identifier [NCT05146804].

Keywords: dietary antiplatelets; immunothrombosis; inflammation; non-communicable diseases; nutrition; phytonutrients; thromboxanes.

Associated data

ClinicalTrials.gov/NCT05146804