Epidemiology of Clinically Significant Aspergillus Species from a Large Tertiary Hospital in Shanghai, China, for the Period of Two Years

Yuyi Zhang; Suzhen Wang; Chunmei Zhou; Yao Zhang; Jue Pan; Baishen Pan; Beili Wang; Bijie Hu; Wei Guo

doi:10.2147/IDR.S417840

Epidemiology of Clinically Significant Aspergillus Species from a Large Tertiary Hospital in Shanghai, China, for the Period of Two Years

Infect Drug Resist. 2023 Jul 17:16:4645-4657. doi: 10.2147/IDR.S417840. eCollection 2023.

Authors

Yuyi Zhang¹, Suzhen Wang¹, Chunmei Zhou¹, Yao Zhang², Jue Pan², Baishen Pan¹, Beili Wang¹, Bijie Hu², Wei Guo¹

Affiliations

¹ Department of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.
² Department of Infectious Disease, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China.

Abstract

Background: Aspergillus species are becoming a major public health concern worldwide due to the increase in the incidence of aspergillosis and emergence of antifungal resistance. In this study, we surveyed all Aspergillus species isolated from aspergillosis patients in Zhongshan Hospital Fudan University, Shanghai, China, from 2019 to 2021.

Methods: We characterized the susceptibility profiles of these Aspergillus species to medical azoles (voriconazole, itraconazole and posaconazole) using YeastOne^TM broth microdilution system. To determine the underlying antifungal resistance mechanisms in azole-resistant A. fumigatus (ARAf) isolates, we characterized mutations in the cyp51A gene. Genotypic diversity of sampled A. fumigatus was investigated using CSP-typing.

Results: A total of 112 Aspergillus isolates (81 A. fumigatus, 17 A. flavus, 5 A. niger, 2 A. terreus, 2 A. lentulus, 2 A. oryzae, 1 A. nidulans, 1 A. versicolor and 1 A. sydowii) from 105 patients diagnosed with aspergillosis (including proven or probable invasive aspergillosis, chronic pulmonary aspergillosis, allergic bronchopulmonary aspergillosis and cutaneous aspergillosis) were obtained. Eight isolates (7 A. fumigatus and 1 A. niger) from seven patients were either azole non-susceptible or non-wild type. Azole non-susceptible or non-wild type rate was 7.1%/isolate and 6.7%/patient analysed. Four ARAf harbored TR34/L98H mutation, whereas one carried TR46/Y121F/T289A allele. The 81 A. fumigatus isolates were spread across 8 CSP types with t01 to be the predominant type (53.1%). ARAf isolates were distributed over CSP types t01, t02, t04A and t11.

Conclusion: Results from this study provided us with an understanding of the antifungal resistance and related characteristics of Aspergillus species in Eastern China. Further comparisons of our results with those in other countries reflect potential clonal expansion of A. fumigatus in our region. Further surveillance study is warranted to guide antifungal therapy and for epidemiological purposes.

Keywords: Aspergillus fumigatus; CSP-typing; antifungal susceptibility profile; aspergillosis; non-susceptible; non-wild type.

Grants and funding

This work was supported by research grants from the Zhongshan Hospital, Fudan University (2021ZSQN41), to Yuyi Zhang.