Quantification of Nτ -Methylhistidine and Nπ-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Jun-Ichi Shiraishi; Daichi Ijiri; Ayumi Katafuchi; Shozo Tomonaga; Saki Shimamoto; Hanwool Do; Shinya Ishihara; Akira Ohtsuka

doi:10.2141/jpsa.2023017

Quantification of N^τ -Methylhistidine and N^π-Methylhistidine in Chicken Plasma by Liquid Chromatography-Tandem Mass Spectrometry

J Poult Sci. 2023 Jul 22:60:2023017. doi: 10.2141/jpsa.2023017. eCollection 2023.

Authors

Jun-Ichi Shiraishi¹, Daichi Ijiri^{2

3}, Ayumi Katafuchi², Shozo Tomonaga⁴, Saki Shimamoto⁵, Hanwool Do⁴, Shinya Ishihara¹, Akira Ohtsuka^{2

3}

Affiliations

¹ Graduate School of Applied Life Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino, Tokyo 180-8602, Japan.
² Graduate School of Agriculture, Forestry and Fisheries, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
³ The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
⁴ Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
⁵ Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2-no-cho, Nishi-ku, Niigata 950-2181, Japan.

Abstract

The concentration of N^τ-methylhistidine in plasma provides an index of skeletal muscle protein breakdown. This study aimed to establish a quantitative method for measuring the concentrations of N^τ-methylhistidine and its isomer N^π-methylhistidine in chicken plasma, using liquid chromatography-tandem mass spectrometry with stable isotope dilution analysis. The acceptable linear ranges of detection were 1.56-50.00 μmol/L for N^τ-methylhistidine and 0.78-25.00 μmol/L for N^π-methylhistidine. The proposed method detected changes in the plasma levels of N^τ-methylhistidine and N^π-methylhistidine in response to fasting and re-feeding. These results suggest that the method developed in this study can be used for the simultaneous measurement of N^τ-methylhistidine and N^π-methylhistidine in chicken plasma.

Keywords: LC–MS/MS; Nπ-methylhistidine; Nτ-methylhistidine; method validation.

2023 Japan Poultry Science Association.