First report of New Delhi metallo-β-lactamase-1-producing Acinetobacter soli in Japan

Michiko Furugaito; Masaki Anraku; Ryuji Kawahara; Akihiro Hisato; Toshinori Kamisako; Koichiro Yoshida

doi:10.1016/j.jiac.2023.07.009

First report of New Delhi metallo-β-lactamase-1-producing Acinetobacter soli in Japan

J Infect Chemother. 2023 Dec;29(12):1177-1180. doi: 10.1016/j.jiac.2023.07.009. Epub 2023 Jul 21.

Authors

Michiko Furugaito¹, Masaki Anraku², Ryuji Kawahara², Akihiro Hisato³, Toshinori Kamisako⁴, Koichiro Yoshida³

Affiliations

¹ Department of Clinical Laboratory, Kindai University Hospital, 377-2, Ohno-Higashi, OsakaSayama, Osaka, 589-8511, Japan. Electronic address: michiko-furugaito@med.kindai.ac.jp.
² Division of Microbiology, Bacteriology Section, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka-shi, Osaka, 537-0025, Japan.
³ Division of Infection Control and Prevention, Department of Medical safety management, Kindai University Hospital, 377-2, Ohno-Higashi, OsakaSayama, Osaka, 589-8511, Japan.
⁴ Department of Clinical Laboratory Medicine, Kindai University Faculty of Medicine, 377-2, Ohno-Higashi, OsakaSayama, Osaka, 589-8511, Japan.

PMID: 37482191
DOI: 10.1016/j.jiac.2023.07.009

Abstract

New Delhi metallo-β-lactamase (NDM)-producing gram-negative rods, including Acinetobacter species, are a global problem but have rarely been isolated in Japan. To our knowledge, this is the first study to isolate an NDM-1-producing Acinetobacter soli strain, KUH106, in Japan. We analyzed this strain using next-generation sequencing to examine the plasmid carrying NDM-1. This plasmid, named pKUH106_NDM1, is 41,135 bp in length and contains genetic contexts with the structure ISAba14-aph(3')-VI-ISAba125-bla_NDM-1ble-_MBL. Comparative analysis of the plasmid revealed that it resembled the plasmids of Acinetobacter detected in various countries, such as the A. soli isolate from Taiwan and the Acinetobacter baumannii isolate from a healthcare facility in Osaka Prefecture, Japan. These results suggest that bla_NDM-1 may spread via this plasmid in Acinetobacter species. This phenomenon needs to be confirmed through the genetic analysis of A. baumannii and other carbapenem-resistant Acinetobacter species. In particular, bla_NDM-1 and other resistance genes must be investigated, and the spread of these genes in the community must be cautioned.

Keywords: Acinetobacter soli; Antimicrobial resistance gene; Carbapenemase; New Delhi metallo-β-lactamase-1; Plasmid.

Copyright © 2023 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. All rights reserved.