A near-infrared fluorescent probe for tracking endogenous and exogenous H2O2 in cells and zebrafish

Yuanyuan Wu; Fengyang Jing; Hanling Huang; Haijie Wang; Shijun Chen; Wenkang Fan; Yiyi Li; Lin Wang; Yaping Wang; Shicong Hou

doi:10.1016/j.saa.2023.123158

A near-infrared fluorescent probe for tracking endogenous and exogenous H₂O₂ in cells and zebrafish

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5:302:123158. doi: 10.1016/j.saa.2023.123158. Epub 2023 Jul 16.

Authors

Affiliations

¹ College of Science, China Agricultural University, Beijing 100193, PR China.
² College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
³ College of Science, China Agricultural University, Beijing 100193, PR China. Electronic address: houshc@cau.edu.cn.

PMID: 37478761
DOI: 10.1016/j.saa.2023.123158

Abstract

H₂O₂ is an important signaling molecule in the body, and its levels fluctuate in many pathological sites, therefore, it can be used as a biomarker for early diagnosis of disease. Since the environment in vivo is extremely complex, it is of great significance to develop a probe that can accurately monitor the fluctuation of H₂O₂ level without interference from other physiological processes. Based on this, we designed and synthesized two new near-infrared H₂O₂ fluorescent probes, LTA and LTQ, based on the ICT mechanism. Both of them have good responses to H₂O₂, but LTA has a faster response speed. In addition, the probe LTA has good biocompatibility, good water solubility, and a large Stokes shift (95 nm). The detection limit is 4.525 μM. The probe was successfully used to visually detect H₂O₂ in living cells and zebrafish and was successfully used to monitor the changes in H₂O₂ levels in zebrafish due to APAP-induced liver injury.

Keywords: Fluorescent probe; Hydrogen peroxide; Live cells; Liver injury.

MeSH terms

Animals
Fluorescent Dyes*
HeLa Cells
Humans
Hydrogen Peroxide
Zebrafish*

Substances

Fluorescent Dyes
Hydrogen Peroxide