Long non-coding RNA CASC2 regulates osteoblasts matrix mineralization

Jaime Freitas; Sara Reis Moura; Mário Adolfo Barbosa; Susana G Santos; Maria Inês Almeida

doi:10.3389/fbioe.2023.1155596

Long non-coding RNA CASC2 regulates osteoblasts matrix mineralization

Front Bioeng Biotechnol. 2023 Jul 4:11:1155596. doi: 10.3389/fbioe.2023.1155596. eCollection 2023.

Authors

Jaime Freitas^{1

2}, Sara Reis Moura^{1

2

3}, Mário Adolfo Barbosa^{1

2}, Susana G Santos^{1

2}, Maria Inês Almeida^{1

2

3}

Affiliations

¹ i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
² INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal.
³ ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.

Abstract

Long non-coding RNAs (lncRNAs) are master regulators of gene expression and have recently emerged as potential innovative therapeutic targets. The deregulation of lncRNA expression patterns has been associated with age-related and noncommunicable diseases in the bone tissue, including osteoporosis and tumors. However, the specific role of lncRNAs in physiological or pathological conditions in the bone tissue still needs to be further clarified, for their exploitation as therapeutic tools. In the present study, we evaluate the potential of the lncRNA CASC2 as a regulator of osteogenic differentiation and mineralization. Results show that CASC2 expression is decreased during osteogenic differentiation of human bone marrow-derived Mesenchymal Stem/Stromal cells (hMSCs). CASC2 knockdown, using small interfering RNA against CASC2 (siCASC2), increases the expression of the late osteogenic marker Bone Sialoprotein (BSP), but does not impact ALP staining level nor the expression of early osteogenic transcripts, including RUNX2 and OPG. Although siCASC2 does not impact hMSC proliferation nor apoptosis, it promotes the mineralization of hMSC cultured under osteogenic-inducing conditions, as shown by the increase of calcium deposits. Mass spectrometry-based proteomic analysis revealed that 89 proteins are regulated by CASC2 at late osteogenic stages, including proteins associated with bone diseases or anthropometric and musculoskeletal traits. Specifically, the Cartilage Oligomeric Matrix Protein (COMP) is highly enhanced by CASC2 knockdown at late stages of osteogenic differentiation, at both transcriptional and protein level. On the other hand, inhibition of COMP impairs osteoblasts mineralization as well as the expression of BSP. The results indicate that lncRNA CASC2 regulates late osteogenic differentiation and mineralization in hMSC via COMP and BSP. In conclusion, this study suggests that targeting lncRNA CASC2 could be a potential approach for modulating bone mineralization.

Keywords: bone; cell differentiation; extracellular matrix; long noncoding transcripts; mesenchymal stem/stromal cells.

Grants and funding

The project was supported by Portuguese funds through FCT-Fundação para a Ciência e a Tecnologia (FCT)/Ministério da Ciência, Tecnologia e Ensino Superior in the framework of the project POCI-01-0145-FEDER-031402, R2Bone (FEDER-Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020-Operacional Programme for Competitiveness and Internationalisation—POCI, Portugal 2020); and by the AO CMF, AOCMFS-21-23A (AO Foundation). SM and MA are supported by FCT (SFRH/BD/147229/2019 and BiotechHealth Program; CEECINST/00091/2018/CP1500/CT0011, respectively).