Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Ellen M Quardokus; Diane C Saunders; Elizabeth McDonough; John W Hickey; Christopher Werlein; Christine Surrette; Presha Rajbhandari; Anna Martinez Casals; Hua Tian; Lisa Lowery; Elizabeth K Neumann; Frida Björklund; Taruna V Neelakantan; Josh Croteau; Anne E Wiblin; Jeremy Fisher; April J Livengood; Karen G Dowell; Jonathan C Silverstein; Jeffrey M Spraggins; Gloria S Pryhuber; Gail Deutsch; Fiona Ginty; Garry P Nolan; Simon Melov; Danny Jonigk; Michael A Caldwell; Ioannis S Vlachos; Werner Muller; Nils Gehlenborg; Brent R Stockwell; Emma Lundberg; Michael P Snyder; Ronald N Germain; Jeannie M Camarillo; Neil L Kelleher; Katy Börner; Andrea J Radtke

doi:10.1038/s41592-023-01846-7

Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Nat Methods. 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7. Epub 2023 Jul 19.

Authors

Ellen M Quardokus^#¹, Diane C Saunders^#^{2

3}, Elizabeth McDonough⁴, John W Hickey⁵, Christopher Werlein⁶, Christine Surrette⁴, Presha Rajbhandari⁷, Anna Martinez Casals^{5

8

9}, Hua Tian¹⁰, Lisa Lowery⁴, Elizabeth K Neumann^{11

12

13}, Frida Björklund⁸, Taruna V Neelakantan¹⁴, Josh Croteau¹⁵, Anne E Wiblin¹⁶, Jeremy Fisher¹⁷, April J Livengood¹⁸, Karen G Dowell¹⁹, Jonathan C Silverstein²⁰, Jeffrey M Spraggins^{11

12

21

22}, Gloria S Pryhuber²³, Gail Deutsch²⁴, Fiona Ginty⁴, Garry P Nolan⁵, Simon Melov²⁵, Danny Jonigk^{6

26

27}, Michael A Caldwell²⁸, Ioannis S Vlachos^{29

30

31}, Werner Muller^{32

33}, Nils Gehlenborg³⁴, Brent R Stockwell^{7

14}, Emma Lundberg^{5

8

9}, Michael P Snyder³⁵, Ronald N Germain³⁶, Jeannie M Camarillo^{28

37}, Neil L Kelleher^#²⁸, Katy Börner^#¹, Andrea J Radtke³⁸

Affiliations

¹ Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.
² Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
³ Vanderbilt Diabetes Center, Vanderbilt University School of Medicine, Nashville, TN, USA.
⁴ GE Research, Niskayuna, NY, USA.
⁵ Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
⁶ Institute of Pathology, Hannover Medical School, Hannover, Germany.
⁷ Department of Biological Sciences, Columbia University, New York, NY, USA.
⁸ Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.
⁹ Department of Bioengineering, Stanford University, Stanford, CA, USA.
¹⁰ Department of Chemistry, Pennsylvania State University, University Park, PA, USA.
¹¹ Department of Biochemistry, Vanderbilt University, Nashville, TN, USA.
¹² Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, USA.
¹³ Department of Chemistry, University of California Davis, Davis, CA, USA.
¹⁴ Department of Chemistry, Columbia University, New York, NY, USA.
¹⁵ Department of Business Development, BioLegend Inc., San Diego, CA, USA.
¹⁶ Department of Research and Development, Abcam PLC, Discovery Drive, Cambridge Biomedical Campus, Cambridge, UK.
¹⁷ Department of Research and Development, Cell Signaling Technology, Inc., Danvers, MA, USA.
¹⁸ Department of Protein and Cell Analysis, Thermo Fisher Scientific, Carlsbad, CA, USA.
¹⁹ Akoya Biosciences, Marlborough, MA, USA.
²⁰ Department of Biomedical Informatics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
²¹ Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
²² Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.
²³ Department of Pediatrics, University of Rochester Medical Center, Rochester, NY, USA.
²⁴ Department of Pathology, University of Washington Medical Center, Seattle, WA, USA.
²⁵ Buck Institute for Research on Aging, Novato, CA, USA.
²⁶ German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
²⁷ Institute of Pathology, RWTH University of Aachen, Aachen, Germany.
²⁸ Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
²⁹ Spatial Technologies Unit, Harvard Medical School Initiative for RNA Medicine, Beth Israel Deaconess Medical Center, Boston, MA, USA.
³⁰ Department of Pathology, Beth Israel Deaconess Medical Center, Boston, MA, USA.
³¹ Broad Institute of MIT and Harvard, Cambridge, MA, USA.
³² Miltenyi Biotec B.V. and Co. KG, Bergisch Gladbach, Germany.
³³ Division of Infection, Immunity and Respiratory Medicine, University of Manchester, Manchester, UK.
³⁴ Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.
³⁵ Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
³⁶ Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH, Bethesda, MD, USA.
³⁷ Inotiv, Nashville, TN, USA.
³⁸ Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH, Bethesda, MD, USA. andrea.radtke@nih.gov.

^# Contributed equally.

Abstract

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural

MeSH terms

Antibodies*
Community Resources*
Diagnostic Imaging
Humans
Reproducibility of Results

Substances

Antibodies

Abstract

Publication types

MeSH terms

Substances

Grants and funding