The Level of Anti-Polyethylene Glycol (PEG) Immunoglobulin G Antibodies in Human Serum Does Not Correlate with Efficiency of PEGylated Nanoparticle Uptake by Monocytes

K V Nevskaya; S V Krivoshchekov; A P Zima; A N Baikov; A M Gur'ev; A G Pershina

doi:10.1007/s10517-023-05848-5

The Level of Anti-Polyethylene Glycol (PEG) Immunoglobulin G Antibodies in Human Serum Does Not Correlate with Efficiency of PEGylated Nanoparticle Uptake by Monocytes

Bull Exp Biol Med. 2023 Jun;175(2):270-274. doi: 10.1007/s10517-023-05848-5. Epub 2023 Jul 19.

Authors

K V Nevskaya¹, S V Krivoshchekov¹, A P Zima¹, A N Baikov¹, A M Gur'ev¹, A G Pershina²

Affiliations

¹ Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
² Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia. allysyz@mail.ru.

PMID: 37466857
DOI: 10.1007/s10517-023-05848-5

Abstract

The presence of anti-polyethylene glycol (PEG) antibodies can limit the clinical efficacy of PEGylated drugs and cause anaphylactic reactions in patients. Monocytes/macrophages are effector cells involved in IgG-mediated passive systemic anaphylaxis. We studied the influence of human blood serum on the efficiency of uptake of PEGylated nanoparticles by human blood monocytes. It has been shown that magnetic nanoparticles modified with PEG-3000 and solid lipid nanoparticles containing PEG-2000 are avidly internalized by human blood monocytes in vitro, the uptake efficiency depends on the features (composition) of donor blood serum, but does not correlate with the level of the IgG antibody against PEG.

Keywords: anti-PEG IgG antibody; magnetic nanoparticles; monocytes; polyethylene glycol; solid lipid nanoparticles.

MeSH terms

Humans
Immunoglobulin G
Monocytes
Nanoparticles*
Polyethylene Glycols
Serum*

Substances

Immunoglobulin G
Polyethylene Glycols