Female solitary bees flexibly change foraging behaviour according to their floral resource requirements and foraging experiences

Abstract

Intraspecific variation in foraging behaviour is related to the floral resource requirements and foraging experiences of social bees. These behavioural changes influence their pollination efficiency. However, the extent of such behavioural changes in solitary bees, which constitute the majority of bee species, remains largely unknown. As pollen contains essential nutrients for ovarian and offspring development, a relationship between the resource requirements of female bees and their ovarian development is expected. Additionally, wing damage could reflect foraging experiences, as the wings are damaged during foraging. Here, we aimed to clarify the relationships between ovarian development, wing damage, foraging behaviours, and pollination efficiency in female long-horned bees (Eucera nipponensis and Eucera spurcatipes) visiting red clovers. The bee handling times were recorded. Wing damage and pollen load on the hind legs were confirmed and the number of pollen grains on bee's bodies was counted. We then dissected the bees and recorded the presence or absence of nectar and pollen in the digestive tubes, as well as the mature egg number. The mature egg number positively correlated with nectar feeding and pollen collection, whereas handling time decreased with wing damage. Bees with pollen loads on their legs attach more pollen grains to their bodies. Therefore, solitary bees flexibly change their foraging behaviour based on resource requirements and foraging experiences, and these behavioural changes can influence pollination efficiency. The asynchrony of foraging behaviours and pollination efficiency within a bee population may provide stable pollination for flowering plants throughout the season.

Keywords: Behavioural change; Eucera bees; Experience; Intraspecies variation; Ovarian development.