The COPII subunit CsSEC23 mediates fruit glossiness in cucumber

Luyao Gao; Jiajian Cao; Siyu Gong; Ning Hao; Yalin Du; Chunhua Wang; Tao Wu

doi:10.1111/tpj.16389

The COPII subunit CsSEC23 mediates fruit glossiness in cucumber

Plant J. 2023 Oct;116(2):524-540. doi: 10.1111/tpj.16389. Epub 2023 Jul 26.

Authors

Luyao Gao^#^{1

2

3}, Jiajian Cao^#^{1

2

3

4}, Siyu Gong⁵, Ning Hao^{1

6}, Yalin Du^{1

2

3

4}, Chunhua Wang^{1

2

3

4}, Tao Wu^{1

2

3

4}

Affiliations

¹ College of Horticulture, Hunan Agricultural University, Changsha, 410128, China.
² Key Laboratory for Evaluation and Utilization of Gene Resources of Horticultural Crops (vegetables, tea, etc.), Ministry of Agriculture and Rural Affairs of China, Changsha, 410128, China.
³ Yuelushan Lab, Changsha, 410128, China.
⁴ Whampoa Innovation Research Institute, Hunan Agricultural University, Changsha, 410128, China.
⁵ College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China.
⁶ Laboratory of Plant Nutrition and Fertilizers, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan.

^# Contributed equally.

PMID: 37460197
DOI: 10.1111/tpj.16389

Abstract

To improve our understanding of the mechanism underlying cucumber glossiness regulation, a novel cucumber mutant with a glossy peel (Csgp) was identified. MutMap, genotyping, and gene editing results demonstrated that CsSEC23, which is the core component of COPII vesicles, mediates the glossiness of cucumber fruit peel. CsSEC23 is functionally conserved and located in the Golgi and endoplasmic reticulum. CsSEC23 could interact with CsSEC31, but this interaction was absent in the Csgp mutant, which decreased the efficiency of COPII vesicle transportation. Genes related to wax and cutin transport were upregulated in the Csgp mutant, and the cuticle structure of the Csgp-mutant peel became thinner. Moreover, the wax and cutin contents were also changed due to CsSEC23 mutation. Taken together, the results obtained from this study revealed that CsSEC23 mediates cucumber glossiness, and this mediating might be affected by COPII vesicle transportation.

Keywords: COPII; CsSEC23; cucumber; glossiness.

Abstract

Grants and funding