Histologic changes of porcine portal vein anastomosis after electrochemotherapy with bleomycin

Urban Stupan; Maja Čemažar; Blaž Trotovšek; Miha Petrič; Aleš Tomažič; Gorana Gašljević; Branislava Ranković; Alenka Seliškar; Tanja Plavec; Jerneja Sredenšek; Jan Plut; Marina Štukelj; Urša Lampreht Tratar; Tanja Jesenko; Alenka Nemec Svete; Gregor Serša; Mihajlo Đokić

doi:10.1016/j.bioelechem.2023.108509

Histologic changes of porcine portal vein anastomosis after electrochemotherapy with bleomycin

Bioelectrochemistry. 2023 Dec:154:108509. doi: 10.1016/j.bioelechem.2023.108509. Epub 2023 Jul 13.

Authors

Urban Stupan¹, Maja Čemažar², Blaž Trotovšek³, Miha Petrič³, Aleš Tomažič³, Gorana Gašljević⁴, Branislava Ranković⁵, Alenka Seliškar⁶, Tanja Plavec⁶, Jerneja Sredenšek⁶, Jan Plut⁶, Marina Štukelj⁶, Urša Lampreht Tratar⁴, Tanja Jesenko⁴, Alenka Nemec Svete⁶, Gregor Serša⁷, Mihajlo Đokić³

Affiliations

¹ University of Ljubljana, Faculty of Medicine, Korytkova ulica 2, SI-1000 Ljubljana, Slovenia. Electronic address: urban.stupan@kclj.si.
² Institute of Oncology Ljubljana, Zaloška cesta 2, SI-1000 Ljubljana, Slovenia; University of Ljubljana, Veterinary Faculty, Gerbičeva ulica 60, SI-1000 Ljubljana, Slovenia.
³ University of Ljubljana, Faculty of Medicine, Korytkova ulica 2, SI-1000 Ljubljana, Slovenia; University Medical Centre Ljubljana, Zaloška cesta 2, SI-1000 Ljubljana, Slovenia.
⁴ Institute of Oncology Ljubljana, Zaloška cesta 2, SI-1000 Ljubljana, Slovenia.
⁵ University of Ljubljana, Faculty of Medicine, Korytkova ulica 2, SI-1000 Ljubljana, Slovenia.
⁶ University of Primorska, Faculty of Health Sciences, Polje 42, SI-6310 Izola, Slovenia.
⁷ Institute of Oncology Ljubljana, Zaloška cesta 2, SI-1000 Ljubljana, Slovenia; University of Ljubljana, Faculty of Health Sciences, Zdravstvena pot 5, SI-1000 Ljubljana, Slovenia.

PMID: 37459749
DOI: 10.1016/j.bioelechem.2023.108509

Abstract

Electrochemotherapy (ECT¹) is used for treatment of unresectable abdominal malignancies. This study aims to show that ECT of porcine portal vein anastomosis is safe and feasible in order to extend the indications for margin attenuation after resection of locally advanced pancreatic carcinoma. No marked differences were found between the control group and ECT treated groups. Electroporation thus caused irreversible damage to the vascular smooth muscle cells in tunica media that could bedue to the narrow irreversible electroporation zone that may occur near the electrodes, or due to vasa vasorum thrombosis in the tunica externa. Based on the absence of vascular complications, and similar histological changes in lienal veinanastomosis, we can conclude that ECT of portal vein anastomosis is safe and feasible.

Keywords: Electrochemotherapy; Pancreatic cancer; Portal vein anastomosis.

MeSH terms

Anastomosis, Surgical
Animals
Bleomycin
Electrochemotherapy*
Pancreatic Neoplasms* / drug therapy
Portal Vein / surgery
Swine

Substances

Bleomycin