FBXO5 acts as a novel prognostic biomarker for patients with cervical cancer

Shan Jiang; Jianfeng Zheng; Zhaolei Cui; Yanhong Li; Qiaoling Wu; Xintong Cai; Chaoqiang Zheng; Yang Sun

doi:10.3389/fcell.2023.1200197

FBXO5 acts as a novel prognostic biomarker for patients with cervical cancer

Front Cell Dev Biol. 2023 Jun 28:11:1200197. doi: 10.3389/fcell.2023.1200197. eCollection 2023.

Authors

Shan Jiang^{1

2}, Jianfeng Zheng¹, Zhaolei Cui³, Yanhong Li^{1

2}, Qiaoling Wu¹, Xintong Cai¹, Chaoqiang Zheng³, Yang Sun¹

Affiliations

¹ Department of Gynecology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.
² College of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
³ Laboratory of Biochemistry and Molecular Biology Research, Department of Clinical Laboratory, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China.

Abstract

Background: Cervical cancer (CC) remains one of the most common and deadly malignancies in women worldwide. FBXO5, a protein-coding gene, is highly expressed in a variety of primary tumors and promotes tumor progression, however, its role and prognostic value in CC remain largely unknown. Methods: A key differential gene, FBXO5, was screened according to WGCNA based on immunohistochemical assays of clinical samples, multiple analyses of the Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases, including survival analysis, tumor mutational burden, GO, KEGG, tumor immune infiltration, and chemotherapeutic drug sensitivity, to explore the expression and prognostic value of FBXO5 in CC. The migration and invasiveness of cervical cancer cells following FBXO5 knockdown and overexpression were examined using wound healing and transwell assays, and the viability of cancer cells was assessed using CCK8 and EdU assays. Results: FBXO5 was discovered to be substantially expressed in CC tissues using data from our CC cohort and the TCGA database, and a survival analysis indicated FBXO5 as a predictive factor for poor overall survival in CC patients. In vitro, CC cells were more inclined to proliferate, migrate, and invade when FBXO5 was upregulated as opposed to when it was knocked down.

Keywords: WGCNA; autophagy; cervical cancer; immunity; prognosis.

Grants and funding

This work was supported by the National Natural Science Foundation of China (81873045), Joint Funds for the Innovation of Science and Technology of Fujian Province (2021Y9209) and the High-level Talents Training Project of Fujian Cancer Hospital (2022YNG04).