Functional roles of two novel P450 genes in the adaptability of Conogethes punctiferalis to three commonly used pesticides

Xingxing Yuan; Han Li; Xianru Guo; He Jiang; Qi Zhang; Lijuan Zhang; Gaoping Wang; Weizheng Li; Man Zhao

doi:10.3389/fphys.2023.1186804

Functional roles of two novel P450 genes in the adaptability of Conogethes punctiferalis to three commonly used pesticides

Front Physiol. 2023 Jun 28:14:1186804. doi: 10.3389/fphys.2023.1186804. eCollection 2023.

Authors

Xingxing Yuan¹, Han Li¹, Xianru Guo¹, He Jiang², Qi Zhang¹, Lijuan Zhang¹, Gaoping Wang¹, Weizheng Li¹, Man Zhao¹

Affiliations

¹ Henan International Laboratory for Green Pest Control, College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
² State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Abstract

Introduction: Insect cytochrome P450 (CYP450) genes play important roles in the detoxification and metabolism of xenobiotics, such as plant allelochemicals, mycotoxins and pesticides. The polyphagous Conogethes punctiferalis is a serious economic pest of fruit trees and agricultural crops, and it shows high adaptability to different living environments. Methods: The two novel P450 genes CYP6CV1 and CYP6AB51 were identified and characterized. Quantitative real-time PCR (qRT-PCR) technology was used to study the expression patterns of the two target genes in different larval developmental stages and tissues of C. punctiferalis. Furthermore, RNA interference (RNAi) technology was used to study the potential functions of the two P450 genes by treating RNAi-silenced larvae with three commonly used pesticides. Results: The CYP6CV1 and CYP6AB51 genes were expressed throughout various C. punctiferalis larval stages and in different tissues. Their expression levels increased along with larval development, and expression levels of the two target genes in the midgut were significantly higher than in other tissues. The toxicity bioassay results showed that the LC₅₀ values of chlorantraniliprole, emamectin benzoate and lambda-cyhalothrin on C. punctiferalis larvae were 0.2028 μg/g, 0.0683 μg/g and 0.6110 mg/L, respectively. After treating with different concentrations of chlorantraniliprole, emamectin benzoate and lambda-cyhalothrin (LC₁₀, LC₃₀, LC₅₀), independently, the relative expressions of the two genes CYP6CV1 and CYP6AB51 were significantly induced. After the dsRNA injection, the expression profiles of the two CYP genes were reduced 72.91% and 70.94%, respectively, and the mortality rates of the larvae significantly increased when treated with the three insecticides independently at LC₁₀ values. Discussion: In the summary, after interfering with the CYP6CV1 and CYP6AB51 in C. punctiferalis, respectively, the sensitivity of C. punctiferalis to chlorantraniliprole, emamectin benzoate and lambda-cyhalothrin was significantly increased, indicating that the two CYP6 genes were responsible for the adaptability of C. punctiferalis to the three chemical insecticides in C. punctiferalis. The results from this study demonstrated that CYP6CV1 and CYP6AB51 in C. punctiferalis play crucial roles in the detoxification of chlorantraniliprole, emamectin benzoate and lambda-cyhalothrin.

Keywords: Conogethes punctiferalis; RNA interference; adaptability; chemical pesticides; cytochrome P450.

Grants and funding

This study was supported by the National Natural Science Foundation of China (Grant No. 31801735) and the State Key Laboratory of Integrated Management of Pest Insects and Rodents (Grant No. IPM2117).