Self-checking dual-modal aptasensor based on hybrid Z-scheme heterostructure of Zn-defective CdS/ZnS for oxytetracycline detection

Pei Song; Ping Qu; Min Wang; Ai-Jun Wang; Yadong Xue; Li-Ping Mei; Jiu-Ju Feng

doi:10.1016/j.aca.2023.341542

Self-checking dual-modal aptasensor based on hybrid Z-scheme heterostructure of Zn-defective CdS/ZnS for oxytetracycline detection

Anal Chim Acta. 2023 Sep 15:1274:341542. doi: 10.1016/j.aca.2023.341542. Epub 2023 Jun 24.

Authors

Pei Song¹, Ping Qu², Min Wang³, Ai-Jun Wang³, Yadong Xue⁴, Li-Ping Mei⁵, Jiu-Ju Feng⁶

Affiliations

¹ Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China; College of Geography and Environmental Sciences, College of Chemistry and Materials Sciences, Zhejiang Normal University, Jinhua, 321004, China.
² Zhejiang Jinhua Ecological and Environmental Monitoring Center, Jinhua, 321015, China.
³ College of Geography and Environmental Sciences, College of Chemistry and Materials Sciences, Zhejiang Normal University, Jinhua, 321004, China.
⁴ Central Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, 321000, China. Electronic address: xueyadong@zju.edu.cn.
⁵ College of Geography and Environmental Sciences, College of Chemistry and Materials Sciences, Zhejiang Normal University, Jinhua, 321004, China. Electronic address: mei.lp@zjnu.edu.cn.
⁶ College of Geography and Environmental Sciences, College of Chemistry and Materials Sciences, Zhejiang Normal University, Jinhua, 321004, China. Electronic address: jjfeng@zjnu.cn.

PMID: 37455075
DOI: 10.1016/j.aca.2023.341542

Abstract

Electrochemical detection methods have been widely used for trace target detection with satisfactory results. However, most of the existing electrochemical sensors rely only on single signal output, which inevitably suffer from the interference of the complex matrix of real samples. Herein, we proposed a dual-modal aptasensor for oxytetracycline assay with self-checking function by integrating photoelectrochemical (PEC) and electrochemical (EC) signal outputs in one analysis system. Zn-defective CdS/ZnS heterostructure was synthesized and served as the photo-electroactive substrate for constructing the biorecognition process, while methylene blue (MB) was used as a dual-functional probe to enhance both PEC and EC signals. Due to the high activity of Zn-defective CdS/ZnS heterojunction and the unique dual-modal signal readout strategy, the biosensing platform exhibits superior analytical performance with the relatively wide linear range (0.01-50 ng mL^-1), lower detection limits of 1.86 pg mL^-1 (PEC mode) and 3.08 pg mL^-1 (EC mode), as well as good selectivity, stability and reproducibility. The proposed dual-model analytical system with self-checking function is envisioned to provide a new approach for sensitive and reliable biosensing.

Keywords: Dual-modal aptasensor; Hybrid Z-scheme heterojunction; Self-checking; Zn-defective.

MeSH terms

Aptamers, Nucleotide* / chemistry
Biosensing Techniques* / methods
Electrochemical Techniques / methods
Limit of Detection
Oxytetracycline*
Reproducibility of Results
Zinc

Substances

zinc sulfide
Oxytetracycline
Zinc
Aptamers, Nucleotide