Comprehensive landscape of the T and B-cell repertoires of newly diagnosed gestational diabetes mellitus

Hui Zhu; Zhijia Zhao; Jin Xu; Yanming Chen; Jie Cai; Chaoyi Shi; Liming Zhou; Qiong Zhu; Lindan Ji

doi:10.1016/j.ygeno.2023.110681

Comprehensive landscape of the T and B-cell repertoires of newly diagnosed gestational diabetes mellitus

Genomics. 2023 Sep;115(5):110681. doi: 10.1016/j.ygeno.2023.110681. Epub 2023 Jul 13.

Authors

Hui Zhu¹, Zhijia Zhao², Jin Xu³, Yanming Chen², Jie Cai⁴, Chaoyi Shi⁴, Liming Zhou⁴, Qiong Zhu⁵, Lindan Ji⁶

Affiliations

¹ Department of Internal Medicine, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, PR China.
² School of Public Health, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, PR China.
³ School of Public Health, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, PR China; Zhejiang Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, PR China.
⁴ Center for Reproductive Medicine, Ningbo Women and Children's Hospital, Ningbo, Zhejiang 315211, PR China.
⁵ Department of Pediatrics, Affiliated People's Hospital of Ningbo University, Ningbo, Zhejiang 315040, PR China.
⁶ Zhejiang Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, PR China; Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, School of Medicine, Ningbo, Zhejiang 315211, PR China. Electronic address: jilindan@nbu.edu.cn.

PMID: 37453476
DOI: 10.1016/j.ygeno.2023.110681

Abstract

This study conducted a high-throughput sequencing analysis of the T- and B- repertoires in the newly diagnosed GDM patients and evaluated the association between abnormal adaptive immunity and GDM. The unique TCR CDR3 clonotypes were mildly decreased in GDM patients, and the similarity of TCR V-J distributions was higher in the GDM group. Moreover, the usages of the V gene and V-J pair and the frequency distributions of some CDR3 amino acids (AAs) both in BCR and TCR were significantly different between groups. Moreover, the cytokines including IL-4, IL-6, IFN-γ and IL-17A were synchronously elevated in the GDM cases. Our findings provide a comprehensive view of BCR and TCR repertoires at newly diagnosed GDM patients, revealing the mild reduction in unique TCRB CDR3 sequences and slight alteration of the V gene, V-J combination and CDR3 (AA) usages of BCR and TCR. This work provides deep insight into the mechanism of maternal adaptive immunity in GDM and provides novel diagnostic biomarkers and potential immunotherapy targets for GDM.

Keywords: B cell receptor; Cytokines; Gestational diabetes mellitus; High-throughput sequencing; Immune repertoires; T cell receptor.